A simple method for deriving functional MSCs and applied for osteogenesis in 3D scaffolds

• Published in: Scientific reports Vol. 3; no. 1; p. 2243
• Main Authors: Zou, Lijin, Luo, Yonglun, Chen, Muwan, Wang, Gang, Ding, Ming, Petersen, Charlotte Christie, Kang, Ran, Dagnaes-Hansen, Frederik, Zeng, Yuanlin, Lv, Nonghua, Ma, Qing, Le, Dang Q. S., Besenbacher, Flemming, Bolund, Lars, Jensen, Thomas G., Kjems, Jørgen, Pu, William T., Bünger, Cody
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 22.07.2013; Nature Publishing Group
• Subjects: 631/532/2074; 631/61/54/2295; 631/61/54/990; 692/308/2171; Animals; Biodegradability; Biodegradation; Calcification, Physiologic; CD105 antigen; CD73 antigen; CD90 antigen; Cell Culture Techniques; Cell Differentiation; Cell Lineage; Fibroblasts - cytology; Gene Expression Regulation; Humanities and Social Sciences; Humans; Hyaluronic acid; Immunophenotyping; Induced Pluripotent Stem Cells - cytology; Induced Pluripotent Stem Cells - metabolism; Karyotype; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - metabolism; Mesenchyme; Mice; multidisciplinary; Oct-4 protein; Osteoblasts - cytology; Osteoblasts - metabolism; Osteogenesis; Osteogenesis - physiology; Phenotype; Pluripotency; Polycaprolactone; Polymers; RNA, Messenger - genetics; Science; Stem cells; Time Factors; Tissue Scaffolds; Tumorigenicity
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep02243

We describe a simple method for bone engineering using biodegradable scaffolds with mesenchymal stem cells derived from human induced-pluripotent stem cells (hiPS-MSCs). The hiPS-MSCs expressed mesenchymal markers (CD90, CD73 and CD105), possessed multipotency characterized by tri-lineages differentiation: osteogenic, adipogenic and chondrogenic and lost pluripotency – as seen with the loss of markers OCT3/4 and TRA-1-81 – and tumorigenicity. However, these iPS-MSCs are still positive for marker NANOG. We further explored the osteogenic potential of the hiPS-MSCs in synthetic polymer polycaprolactone (PCL) scaffolds or PCL scaffolds functionalized with natural polymer hyaluronan and ceramic TCP (PHT) both in vitro and in vivo . Our results showed that these iPS-MSCs are functionally compatible with the two 3D scaffolds tested and formed typically calcified structure in the scaffolds. Overall, our results suggest the iPS-MSCs derived by this simple method retain fully osteogenic function and provide a new solution towards personalized orthopedic therapy in the future.