The effect of initial dose on the recovery and final yields of Heterorhabditis megidis (Rhabditida: Heterorhabditidae) in larvae of the great wax moth, Galleria mellonella

• Published in: Acta parasitologica Vol. 59; no. 2; pp. 213 - 218
• Main Authors: Tumialis, Dorota, Pezowicz, Elżbieta, Mazurkiewicz, Anna, Skrzecz, Iwona, Popowska-Nowak, Elżbieta, Petrykowska, Agnieszka
• Format: Journal Article
• Language: English
• Published: Heidelberg Versita 01.06.2014; Springer Nature B.V
• Subjects: Animal Systematics/Taxonomy/Biogeography; Animals; Biomedical and Life Sciences; Biomedicine; Ecology; final yields; Galleria mellonella; Heterorhabditidae; Heterorhabditis megidis; Infections; initial dose; Larva - parasitology; Life sciences; Medical Microbiology; Microbiology; Moths - parasitology; Nematoda; Nematodes; Original Paper; Parasite Load; Parasitology; recovery; Rhabditida; Rhabditoidea - growth & development; Rhabditoidea - isolation & purification; Poland; initial dose; final yields; recovery
• ISSN: 1230-2821; 1896-1851; 1896-1851
• DOI: 10.2478/s11686-014-0231-5

The aim of this study was to determine the effect of different initial doses of the infective juveniles (IJs) (50 IJs, 200 IJs, 1000 IJs) of Heterorhabditis megidis Poinar (Rhabditida: Heterorhabditidae) strain IsM15/09 on recovery, final yields and percent final yields in larvae Galleria mellonella ( L.). Percent recovery was not directly related to initial dose. Final yields also did not change with the initial dose. However, percent yields was highly negatively correlated with initial dose of nematodes and was the highest with the 50 IJs dose. Additional point of the study was to investigate whether the nematodes are able to produce progeny from one hermaphroditic individual. The results showed that the invasive larvae resumed growth and transformed into hermaphroditic individuals that reproduced without cross-fertilisation.