Proteomic analysis of murine testes lipid droplets

• Published in: Scientific reports Vol. 5; no. 1; p. 12070
• Main Authors: Wang, Weiyi, Wei, Suning, Li, Linghai, Su, Xueying, Du, Congkuo, Li, Fengjuan, Geng, Bin, Liu, Pingsheng, Xu, Guoheng
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 10.07.2015; Nature Publishing Group
• Subjects: 13/1; 13/51; 14/19; 14/63; 631/443/494/2732/2730; 64/60; 692/163/2743/1526/1561; 692/163/2743/2730; 82/58; 82/83; Adipocytes; Animals; Biosynthesis; Carrier Proteins - metabolism; Chaperones; Cholesterol; Enzymes; Esterase; Esterases - metabolism; Humanities and Social Sciences; Leydig cells; Leydig Cells - metabolism; Lipase; Lipase - metabolism; Lipid Droplets - metabolism; Lipid metabolism; Lipid Metabolism - physiology; Lipids; Male; Mass spectrometry; Mass spectroscopy; Mice; Mice, Inbred C57BL; multidisciplinary; Perilipin-1; Phosphoproteins - metabolism; Protein composition; Proteome - metabolism; Proteomics - methods; rab GTP-Binding Proteins - metabolism; Science; Steroid hormones; Steroidogenesis; Steroids - metabolism; Testes; Testosterone
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep12070

Testicular Leydig cells contain abundant cytoplasmic lipid droplets (LDs) as a cholesteryl-ester store for releasing cholesterols as the precursor substrate for testosterone biosynthesis. Here, we identified the protein composition of testicular LDs purified from adult mice by using mass spectrometry and immunodetection. Among 337 proteins identified, 144 were previously detected in LD proteomes; 44 were confirmed by microscopy. Testicular LDs contained multiple Rab GTPases, chaperones and proteins involved in glucuronidation, ubiquination and transport, many known to modulate LD formation and LD-related cellular functions. In particular, testicular LDs contained many members of both the perilipin family and classical lipase/esterase superfamily assembled predominately in adipocyte LDs. Thus, testicular LDs might be regulated similar to adipocyte LDs. Remarkably, testicular LDs contained a large number of classical enzymes for biosynthesis and metabolism of cholesterol and hormonal steroids, so steroidogenic reactions might occur on testicular LDs or the steroidogenic enzymes and products could be transferred through testicular LDs. These characteristics differ from the LDs in most other types of cells, so testicular LDs could be an active organelle functionally involved in steroidogenesis.