Microelectrode array stimulation combined with intrinsic optical imaging: A novel tool for functional brain mapping

• Published in: Journal of neuroscience methods Vol. 263; pp. 7 - 14
• Main Authors: Chernov, Mykyta M., Chen, Gang, Torre-Healy, Luke A., Friedman, Robert M., Roe, Anna W.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.04.2016
• Subjects: Animals; Brain Mapping; Cerebral Cortex - diagnostic imaging; Cerebral Cortex - physiology; Cortical mapping; Electric Stimulation; Forelimb - physiology; Functional tract tracing; Macaca mulatta; Microelectrodes; Microstimulation; Movement - physiology; Optical chamber; Optical Imaging; Utah array; Wakefulness; Optical chamber; Utah array; Functional tract tracing; Cortical mapping; Microstimulation
• ISSN: 0165-0270; 1872-678X
• DOI: 10.1016/j.jneumeth.2016.01.018

•Visualize cortical circuitry.•Combined imaging and electrophysiological recording.•Multiple simultaneous or sequential stimulation sites. Functional brain mapping via cortical microstimulation is a widely used clinical and experimental tool. However, data are traditionally collected point by point, making the technique very time consuming. Moreover, even in skilled hands, consistent penetration depths are difficult to achieve. Finally, the effects of microstimulation are assessed behaviorally, with no attempt to capture the activity of the local cortical circuits being stimulated. We propose a novel method for functional brain mapping, which combines the use of a microelectrode array with intrinsic optical imaging. The precise spacing of electrodes allows for fast, accurate mapping of the area of interest in a regular grid. At the same time, the optical window allows for visualization of local neural connections when stimulation is combined with intrinsic optical imaging. We demonstrate the efficacy of our technique using the primate motor cortex as a sample application, using a combination of microstimulation, imaging and electrophysiological recordings during wakefulness and under anesthesia. Comparison with current method: We find the data collected with our method is consistent with previous data published by others. We believe that our approach enables data to be collected faster and in a more consistent fashion and makes possible a number of studies that would be difficult to carry out with the traditional approach. Our technique allows for simultaneous modulation and imaging of cortical sensorimotor networks in wakeful subjects over multiple sessions which is highly desirable for both the study of cortical organization and the design of brain machine interfaces.