Bustos virus, a new member of the negevirus group isolated from a Mansonia mosquito in the Philippines

• Published in: Archives of virology Vol. 162; no. 1; pp. 79 - 88
• Main Authors: Fujita, Ryosuke, Kuwata, Ryusei, Kobayashi, Daisuke, Bertuso, Arlene Garcia, Isawa, Haruhiko, Sawabe, Kyoko
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.01.2017; Springer Nature B.V
• Subjects: Amino acids; Animals; Antibodies; Biomedical and Life Sciences; Biomedicine; Cell culture; Cell Line; coat proteins; Culex; Culicidae - virology; Cytopathogenic Effect, Viral; cytopathogenicity; Female; gene expression; Gene Expression Profiling; Genome, Viral; Genomes; Infections; Infectious Diseases; mammals; Mansonia; Medical Microbiology; Mosquitoes; Open Reading Frames; Original Article; Peptides; Philippines; Proteins; R&D; Research & development; RNA; RNA polymerase; RNA Viruses - classification; RNA Viruses - genetics; RNA Viruses - isolation & purification; RNA Viruses - physiology; RNA, Viral - genetics; Sequence Analysis, DNA; Viral Proteins - analysis; virion; Virology; Virus Cultivation; Virus Replication; Viruses; Philippines; Japan; Virus ORF1; Subgenomic RNAs; Mosquito Pool; Nucleotide Position; Amino Acid Position

We isolated two distinct viruses from mosquitoes collected in Bustos, Bulacan province, Philippines, in 2009. These viruses show rapid replication and strong cytopathic effects in mosquito C6/36 cells. Whole-genome analysis of these viruses demonstrated that both viruses belong to the negevirus grou...