Bustos virus, a new member of the negevirus group isolated from a Mansonia mosquito in the Philippines

• Published in: Archives of virology Vol. 162; no. 1; pp. 79 - 88
• Main Authors: Fujita, Ryosuke, Kuwata, Ryusei, Kobayashi, Daisuke, Bertuso, Arlene Garcia, Isawa, Haruhiko, Sawabe, Kyoko
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.01.2017; Springer Nature B.V
• Subjects: Amino acids; Animals; Antibodies; Biomedical and Life Sciences; Biomedicine; Cell culture; Cell Line; coat proteins; Culex; Culicidae - virology; Cytopathogenic Effect, Viral; cytopathogenicity; Female; gene expression; Gene Expression Profiling; Genome, Viral; Genomes; Infections; Infectious Diseases; mammals; Mansonia; Medical Microbiology; Mosquitoes; Open Reading Frames; Original Article; Peptides; Philippines; Proteins; R&D; Research & development; RNA; RNA polymerase; RNA Viruses - classification; RNA Viruses - genetics; RNA Viruses - isolation & purification; RNA Viruses - physiology; RNA, Viral - genetics; Sequence Analysis, DNA; Viral Proteins - analysis; virion; Virology; Virus Cultivation; Virus Replication; Viruses; Philippines; Japan; Virus ORF1; Subgenomic RNAs; Mosquito Pool; Nucleotide Position; Amino Acid Position
• ISSN: 0304-8608; 1432-8798; 1432-8798
• DOI: 10.1007/s00705-016-3068-4

We isolated two distinct viruses from mosquitoes collected in Bustos, Bulacan province, Philippines, in 2009. These viruses show rapid replication and strong cytopathic effects in mosquito C6/36 cells. Whole-genome analysis of these viruses demonstrated that both viruses belong to the negevirus group. One of the viruses, from Culex vishunui mosquitoes, is a new strain of Negev virus. The other virus, from a Mansonia sp. mosquito, is a new negevirus designated Bustos virus. Gene expression analysis of the Bustos virus revealed that infected cells contain viral subgenomic RNAs that probably include open reading frame (ORF) 2 or ORF3. Purified Bustos virus particles contained at least three proteins, and the major component (a probable major capsid protein) is encoded by ORF3. Bustos virus did not show infectivity in mammalian BHK-21 cells, suggesting that it is an insect-specific virus, like other known negeviruses.