TSP1-producing B cells show immune regulatory property and suppress allergy-related mucosal inflammation

• Published in: Scientific reports Vol. 3; no. 1; p. 3345
• Main Authors: Zhang, Huan-Ping, Wu, Yingying, Liu, Jiangqi, Jiang, Jing, Geng, Xiao-Rui, Yang, Gui, Mo, Lihua, Liu, Zhi-Qiang, Liu, Zhi-Gang, Yang, Ping-Chang
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 26.11.2013; Nature Publishing Group
• Subjects: 13; 13/21; 13/31; 13/89; 631/250; 692/308; Allergic diseases; Allergies; Animals; Antigens; B-Lymphocytes - immunology; B-Lymphocytes - metabolism; B7-1 Antigen - metabolism; B7-2 Antigen - metabolism; CD4 antigen; CD80 antigen; CD86 antigen; Cell culture; Cholera Toxin - immunology; Dendritic cells; Dendritic Cells - immunology; Food allergies; Food Hypersensitivity - immunology; Humanities and Social Sciences; Immune Tolerance - immunology; Immunoregulation; Immunotherapy; Immunotherapy - methods; Inflammation; Inflammation - immunology; Interleukin-13 - metabolism; Interleukin-4 - metabolism; Interleukin-5 - metabolism; Intestine; Intestines - cytology; Intestines - immunology; Lymphocyte Activation - immunology; Lymphocyte receptors; Lymphocytes B; Lymphocytes T; Mice; Mice, Inbred BALB C; Mucosa; multidisciplinary; Ovalbumin - immunology; Receptors, Complement 3b - biosynthesis; Receptors, Complement 3b - immunology; Science; T cell receptors; T-Lymphocytes, Regulatory - cytology; T-Lymphocytes, Regulatory - immunology; Thrombospondin; Thrombospondin 1 - biosynthesis; Thrombospondin 1 - immunology
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep03345

Specific immunotherapy (SIT) is the only specific remedy for the treatment of allergic diseases currently. B cells are important immune cells in the immunity. The role of B cells in immune regulatory activities has not been fully understood yet. This study aims to elucidate the role of the thrombospondin (TSP)1-producing B cells in the immune regulatory role of SIT. The results showed that after SIT, the frequency of CD35 + B cells was increased in the intestine of mice with food allergy. The CD35 + B cells expressed TSP1 after exposure to specific antigens. Co-culture with the TSP1-producing CD35 + B cells decreased the levels of CD80/CD86 in dendritic cells; the cells convert naïve CD4 + T cells to regulatory T cells to inhibit allergic inflammation in the intestine.