Fluorescence-Raman Dual Modal Endoscopic System for Multiplexed Molecular Diagnostics

• Published in: Scientific reports Vol. 5; no. 1; p. 9455
• Main Authors: Jeong, Sinyoung, Kim, Yong-il, Kang, Homan, Kim, Gunsung, Cha, Myeong Geun, Chang, Hyejin, Jung, Kyung Oh, Kim, Young-Hwa, Jun, Bong-Hyun, Hwang, Do Won, Lee, Yun-Sang, Youn, Hyewon, Lee, Yoon-Sik, Kang, Keon Wook, Lee, Dong Soo, Jeong, Dae Hong
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 30.03.2015; Nature Publishing Group
• Subjects: 14/10; 140/133; 639/624/1107/510; 692/699/67/2321; 692/700/1421/164/2224; Animals; Bioluminescence; Breast cancer; Breast Neoplasms - diagnosis; Breast Neoplasms - genetics; Breast Neoplasms - pathology; Cell Line, Tumor; Endoscopy; Epidermal growth factor; Epidermal growth factor receptors; ErbB-2 protein; Female; Fluorescence; Humanities and Social Sciences; Humans; Mice; multidisciplinary; Pathology, Molecular; Receptor, Epidermal Growth Factor - biosynthesis; Receptor, Epidermal Growth Factor - genetics; Receptor, ErbB-2 - biosynthesis; Receptor, ErbB-2 - genetics; Rodents; Science; Spectrometry, Fluorescence; Spectrum Analysis, Raman; Xenograft Model Antitumor Assays; Xenografts
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep09455

Optical endoscopic imaging, which was recently equipped with bioluminescence, fluorescence and Raman scattering, allows minimally invasive real-time detection of pathologies on the surface of hollow organs. To characterize pathologic lesions in a multiplexed way, we developed a dual modal fluorescence-Raman endomicroscopic system (FRES), which used fluorescence and surface-enhanced Raman scattering nanoprobes (F-SERS dots). Real-time, in vivo and multiple target detection of a specific cancer was successful, based on the fast imaging capability of fluorescence signals and the multiplex capability of simultaneously detected SERS signals using an optical fiber bundle for intraoperative endoscopic system. Human epidermal growth factor receptor 2 (HER2) and epidermal growth factor receptor (EGFR) on the breast cancer xenografts in a mouse orthotopic model were successfully detected in a multiplexed way, illustrating the potential of FRES as a molecular diagnostic instrument that enables real-time tumor characterization of receptors during routine endoscopic procedures.