Highly Stable and Sensitive Fluorescent Probes (LysoProbes) for Lysosomal Labeling and Tracking

• Published in: Scientific reports Vol. 5; no. 1; p. 8576
• Main Authors: Yapici, Nazmiye B., Bi, Yue, Li, Pengfei, Chen, Xin, Yan, Xin, Mandalapu, Srinivas Rao, Faucett, Megan, Jockusch, Steffen, Ju, Jingfang, Gibson, K. Michael, Pavan, William J., Bi, Lanrong
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 26.02.2015; Nature Publishing Group
• Subjects: 14; 14/34; 631/1647/245/2225; 692/308/575; Apoptosis; Cancer; Fluorescent Dyes - chemistry; Fluorescent indicators; HeLa Cells; Humanities and Social Sciences; Humans; Hydrogen-Ion Concentration; Labeling; Lactose; Lactose - chemistry; Localization; Lysosomes - chemistry; Lysosomes - physiology; Metastases; multidisciplinary; pH effects; Protein transport; Quantum dots; Retention; Science; Spectrometry, Fluorescence; Staining and Labeling; Stains & staining
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep08576

We report the design, synthesis and application of several new fluorescent probes (LysoProbes I-VI) that facilitate lysosomal pH monitoring and characterization of lysosome-dependent apoptosis. LysoProbes are superior to commercially available lysosome markers since the fluorescent signals are both stable and highly selective and they will aid in characterization of lysosome morphology and trafficking. We predict that labeling of cancer cells and solid tumor tissues with LysoProbes will provide an important new tool for monitoring the role of lysosome trafficking in cancer invasion and metastasis.