Western Diet Triggers NLRP3-Dependent Innate Immune Reprogramming

• Published in: Cell Vol. 172; no. 1-2; pp. 162 - 175.e14
• Main Authors: Christ, Anette, Günther, Patrick, Lauterbach, Mario A.R., Duewell, Peter, Biswas, Debjani, Pelka, Karin, Scholz, Claus J., Oosting, Marije, Haendler, Kristian, Baßler, Kevin, Klee, Kathrin, Schulte-Schrepping, Jonas, Ulas, Thomas, Moorlag, Simone J.C.F.M., Kumar, Vinod, Park, Min Hi, Joosten, Leo A.B., Groh, Laszlo A., Riksen, Niels P., Espevik, Terje, Schlitzer, Andreas, Li, Yang, Fitzgerald, Michael L., Netea, Mihai G., Schultze, Joachim L., Latz, Eicke
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 11.01.2018
• Subjects: Adult; Aged; Animals; ASC; atherosclerosis; Cells, Cultured; Cellular Reprogramming; Diet, Western; Epigenesis, Genetic; epigenetic reprogramming; Female; granulocyte macrophage progenitors; Humans; Immunity, Innate; Immunologic Memory; innate immune memory; Lipoproteins, LDL - metabolism; Male; Mice; Mice, Inbred C57BL; Middle Aged; Myeloid Cells - immunology; NLR Family, Pyrin Domain-Containing 3 Protein - genetics; NLR Family, Pyrin Domain-Containing 3 Protein - metabolism; NLRP3 inflammasome; non-communicable diseases; Quantitative Trait Loci; Receptors, LDL - genetics; sterile inflammation; trained immunity; Western diet feeding; sterile inflammation; atherosclerosis; ASC; granulocyte macrophage progenitors; innate immune memory; non-communicable diseases; Western diet feeding; epigenetic reprogramming; trained immunity; NLRP3 inflammasome
• ISSN: 0092-8674; 1097-4172
• DOI: 10.1016/j.cell.2017.12.013

Long-term epigenetic reprogramming of innate immune cells in response to microbes, also termed “trained immunity,” causes prolonged altered cellular functionality to protect from secondary infections. Here, we investigated whether sterile triggers of inflammation induce trained immunity and thereby influence innate immune responses. Western diet (WD) feeding of Ldlr−/− mice induced systemic inflammation, which was undetectable in serum soon after mice were shifted back to a chow diet (CD). In contrast, myeloid cell responses toward innate stimuli remained broadly augmented. WD-induced transcriptomic and epigenomic reprogramming of myeloid progenitor cells led to increased proliferation and enhanced innate immune responses. Quantitative trait locus (QTL) analysis in human monocytes trained with oxidized low-density lipoprotein (oxLDL) and stimulated with lipopolysaccharide (LPS) suggested inflammasome-mediated trained immunity. Consistently, Nlrp3−/−/Ldlr−/− mice lacked WD-induced systemic inflammation, myeloid progenitor proliferation, and reprogramming. Hence, NLRP3 mediates trained immunity following WD and could thereby mediate the potentially deleterious effects of trained immunity in inflammatory diseases. [Display omitted] •Western diet triggers innate immunity, NOD-like, and IFN signaling pathways•Western diet alters in vivo LPS responses of GMPs•Western diet induces long-lasting trained immunity in myeloid cells•NLRP3 recognizes Western diet and mediates trained immunity Systemic inflammation induced by a Western diet is largely blunted by dietary changes, but myeloid cell-induced innate immune responses remain augmented and could potentially contribute to inflammatory disease.