A single-dose of oral nattokinase potentiates thrombolysis and anti-coagulation profiles

• Published in: Scientific reports Vol. 5; no. 1; p. 11601
• Main Authors: Kurosawa, Yuko, Nirengi, Shinsuke, Homma, Toshiyuki, Esaki, Kazuki, Ohta, Mitsuhiro, Clark, Joseph F., Hamaoka, Takafumi
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 25.06.2015; Nature Publishing Group
• Subjects: 692/308/2778; 692/308/575; Antithrombin; Antithrombins - blood; Blindness; Blood; Blood Coagulation - drug effects; Blood Coagulation Tests; Blood levels; Coagulation; Coagulation factors; Cross-Over Studies; Degradation products; Double-Blind Method; Enzymes; Factor VIII - metabolism; Fibrin; Fibrin - metabolism; Fibrin Fibrinogen Degradation Products - metabolism; Fibrinogen; Fibrinogen - metabolism; Fibrinolysis; Fibrinolysis - drug effects; Fibrinolytic Agents - administration & dosage; Fibrinolytic Agents - pharmacology; Humanities and Social Sciences; Humans; Immunoassay; Laboratories; Male; Males; multidisciplinary; Partial Thromboplastin Time; Science; Subtilisins - administration & dosage; Subtilisins - pharmacology; Thrombolysis; Thrombolytic drugs; Thromboplastin; Time Factors
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep11601

Our aim was to determine the quantitative effects of a single-dose of Nattokinase (NK) administration on coagulation/fibrinolysis parameters comprehensively in healthy male subjects. A double-blind, placebo-controlled cross-over NK intervention study was carried out in 12 healthy young males. Following the baseline blood draw, each subject was randomized to receive either a single-dose of 2,000 FU NK (NSK-SD, Japan Bio Science Laboratory Co., Ltd) or placebo with subsequent cross-over of the groups. Subjects donated blood samples at 2, 4, 6 and 8 hours following administration for analysis of coagulation/fibrinolysis parameters. As a result, D-dimer concentrations at 6 and 8 hours and blood fibrin/fibrinogen degradation products at 4 hours after NK administration elevated significantly (p < 0.05, respectively). Factor VIII activity declined at 4 and 6 hours (p < 0.05, respectively), blood antithrombin concentration was higher at 2 and 4 hours (p < 0.05, respectively) and the activated partial thromboplastin time prolonged significantly at 2 and 4 hours following NK administration (p < 0.05 and p < 0.01, respectively). All the changes, however, were within the normal range. In conclusion, thus, a single-dose of NK administration appears enhancing fibrinolysis and anti-coagulation via several different pathways simultaneously.