SAP30, a Component of the mSin3 Corepressor Complex Involved in N-CoR-Mediated Repression by Specific Transcription Factors

• Published in: Molecular cell Vol. 2; no. 1; pp. 33 - 42
• Main Authors: Laherty, Carol D., Billin, Andrew N., Lavinsky, Robert M., Yochum, Gregory S., Bush, Angela C., Sun, Jian-Min, Mullen, Tina-Marie, Davie, James R., Rose, David W., Glass, Christopher K., Rosenfeld, Michael G., Ayer, Donald E., Eisenman, Robert N.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.07.1998
• Subjects: Amino Acid Sequence; Animals; Cell Line, Transformed; DNA-Binding Proteins - physiology; Histone Deacetylases - genetics; Histone Deacetylases - physiology; Homeodomain Proteins - physiology; Humans; Kidney; Macromolecular Substances; Mice; Models, Genetic; Molecular Sequence Data; Multiprotein Complexes; Nuclear Proteins - physiology; Nuclear Receptor Co-Repressor 1; Receptors, Estrogen - antagonists & inhibitors; Receptors, Retinoic Acid - physiology; Receptors, Thyroid Hormone - physiology; Repressor Proteins - physiology; Saccharomyces cerevisiae Proteins; Transcription Factor Pit-1; Transcription Factors - physiology; Transcription, Genetic; Transfection
• ISSN: 1097-2765; 1097-4164; 1097-4164
• DOI: 10.1016/S1097-2765(00)80111-2

The transcriptional corepressor mSin3 is found in a large multiprotein complex containing the histone deacetylases HDAC1 and HDAC2, in addition to at least five tightly associated polypeptides. We have cloned and characterized a novel component of the mSin3 complex, SAP30. SAP30 binds to mSin3 and is capable of mediating transcriptional repression via histone deacetylases. SAP30 also binds the N-CoR corepressor and is required for N-CoR-mediated repression by antagonist-bound estrogen receptor and the homeodomain protein Rpx, as well as N-CoR suppression of transactivation by the POU domain protein Pit-1. However, SAP30 is not required for N-CoR-mediated repression by unliganded retinoic acid receptor or thyroid hormone receptor, suggesting that SAP30 is involved in the functional recruitment of the mSin3–histone deacetylase complex to a specific subset of N-CoR corepressor complexes.