High expression of microRNA-183/182/96 cluster as a prognostic biomarker for breast cancer

• Published in: Scientific reports Vol. 6; no. 1; p. 24502
• Main Authors: Song, Cailu, Zhang, Lijuan, Wang, Jin, Huang, Zhongying, Li, Xing, Wu, Mingqing, Li, Shuaijie, Tang, Hailin, Xie, Xiaoming
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 13.04.2016; Nature Publishing Group
• Subjects: 13/1; 13/105; 13/51; 14; 14/105; 14/63; 38; 38/90; 631/67/1347; 631/67/1857; Biomarkers; Biomarkers, Tumor - genetics; Breast cancer; Disease Progression; Female; Humanities and Social Sciences; Humans; Mammography; Metastases; MicroRNAs; MicroRNAs - genetics; miRNA; multidisciplinary; Multigene Family; Polymerase chain reaction; Prognosis; Real-Time Polymerase Chain Reaction; Science; Tumor cell lines; Tumorigenesis
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep24502

More sensitive and effective diagnostic markers for the detection of breast cancer are urgently needed. The microRNA-183/182/96 cluster has been reported to be involved in tumorigenesis and progression in a variety of cancers and it is a promising cancer prognostic biomarker. The goal of this study was to determine the expression levels of the miR-183/182/96 cluster in breast cancer tissues and evaluate its prognostic role in breast cancer. Real-time quantitative polymerase chain reaction analysis (qRT-PCR) was used to detect the expression levels of the miR-183/182/96 cluster in 41 breast cancer tissues and adjacent normal tissues (control tissues) and also in different mammary cell lines. In situ hybridization (ISH) of the miR-183/182/96 cluster on 131 tissue microarrays (TMAs) was used to statistically analyze its prognostic role. The miR-183/182/96 cluster levels were significantly higher in breast cancer tissues than in control tissues. The miR-183/182/96 cluster was also upregulated in human breast cancer cell lines. An increased miR-183/182/96 cluster level was correlated with local relapse, distant metastasis and poor clinical outcomes. Our findings improve our understanding of the expression level of the miR-183/182/96 cluster in breast cancer and clarify the role of the miR-183/182/96 cluster as a novel prognostic biomarker for breast cancer.