An endometrial gene expression signature accurately predicts recurrent implantation failure after IVF

The primary limiting factor for effective IVF treatment is successful embryo implantation. Recurrent implantation failure (RIF) is a condition whereby couples fail to achieve pregnancy despite consecutive embryo transfers. Here we describe the collection of gene expression profiles from mid-luteal p...

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Published inScientific reports Vol. 6; no. 1; p. 19411
Main Authors Koot, Yvonne E. M., van Hooff, Sander R., Boomsma, Carolien M., van Leenen, Dik, Groot Koerkamp, Marian J. A., Goddijn, Mariëtte, Eijkemans, Marinus J. C., Fauser, Bart C. J. M., Holstege, Frank C. P., Macklon, Nick S.
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 22.01.2016
Nature Publishing Group
Subjects
38/39
631/114/1386
692/4017
Adult
Biopsy
Embryo Implantation - genetics
Embryos
Endometrium
Endometrium - metabolism
Endometrium - pathology
Female
Fertilization in Vitro
Gene expression
Gene Expression Profiling
Gene Expression Regulation
Humanities and Social Sciences
Humans
Implantation
In vitro fertilization
Infertility, Female - genetics
multidisciplinary
Pregnancy
Recurrence
Reproducibility of Results
Science
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Summary:The primary limiting factor for effective IVF treatment is successful embryo implantation. Recurrent implantation failure (RIF) is a condition whereby couples fail to achieve pregnancy despite consecutive embryo transfers. Here we describe the collection of gene expression profiles from mid-luteal phase endometrial biopsies (n = 115) from women experiencing RIF and healthy controls. Using a signature discovery set (n = 81) we identify a signature containing 303 genes predictive of RIF. Independent validation in 34 samples shows that the gene signature predicts RIF with 100% positive predictive value (PPV). The strength of the RIF associated expression signature also stratifies RIF patients into distinct groups with different subsequent implantation success rates. Exploration of the expression changes suggests that RIF is primarily associated with reduced cellular proliferation. The gene signature will be of value in counselling and guiding further treatment of women who fail to conceive upon IVF and suggests new avenues for developing intervention.
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These authors contributed equally to this work.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep19411