Repeatability, reproducibility and agreement of intraocular pressure measurement in rabbits by the TonoVet and Tono-Pen

• Published in: Scientific reports Vol. 6; no. 1; p. 35187
• Main Authors: Ma, Di, Chen, Chong-Bo, Liang, Jiajian, Lu, Zhihao, Chen, Haoyu, Zhang, Mingzhi
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 12.10.2016; Nature Publishing Group
• Subjects: 692/308/2778; 692/699/3161/3169/3170; Accuracy; Agreements; Cornea; Correlation coefficient; Glaucoma; Humanities and Social Sciences; multidisciplinary; Pressure; Pressure measurement; Rabbits; Reproducibility; Science
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep35187

Tono-Pen and TonoVet have been used in rabbits to measure intraocular pressure (IOP) and investigate the effect of IOP lowering therapies. Therefore, their reliability and accuracy are very important and deserve careful evaluation. Our results showed that the with-subject deviation ( S w ) and intraclass correlation coefficient (ICC) of the TonoVet and Tono-Pen were 0.61 mmHg/0.83 mmHg and 0.97/0.94, respectively for intrasession repeatability. For intersession reproducibility, the Sw and ICC of TonoVet and Tono-Pen were 1.42 mmHg/1.66 mmHg and 0.73/0.67, respectively. For interoperator reproducibility, the S w and ICC of the TonoVet and Tono-Pen were 0.72 mmHg/1.11 mmHg and 0.91/0.82 respectively. Both TonoVet and Tono-Pen underestimated the IOP measured by manometry. The regression function was: y = 0.8249x + 0.1011 and y =0.6881x + 2.2290 for TonoVet and Tono-Pen, respectively. Our study suggests that both TonoVet and Tono-Pen had excellent intrasession repeatability and inter-operator reproducibility, but good intersession reproducibility. Both TonoVet and Tono-Pen correlated well with manometry, but underestimated the manometric IOP with presence of fixed and proportional biases. These factors should be considered when measuring IOP with Tono-Pen or TonoVet in rabbit eyes.