Chondrogenic Potential of Peripheral Blood Derived Mesenchymal Stem Cells Seeded on Demineralized Cancellous Bone Scaffolds

• Published in: Scientific reports Vol. 6; no. 1; p. 36400
• Main Authors: Wang, Shao-Jie, Jiang, Dong, Zhang, Zheng-Zheng, Huang, Ai-Bing, Qi, Yan-Song, Wang, Hai-Jun, Zhang, Ji-Ying, Yu, Jia-Kuo
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 08.11.2016; Nature Publishing Group
• Subjects: 13/100; 13/107; 13/31; 13/51; 14/19; 631/532/2074; 64; 692/308/2171; Animals; Bone marrow; Cancellous bone; Cancellous Bone - cytology; Cartilage (articular); Cell Culture Techniques; Cell Proliferation; Cells, Cultured; Chondrocytes; Chondrocytes - cytology; Chondrogenesis; Collagen Type II - genetics; Collagen Type II - metabolism; Collagenase 3; Humanities and Social Sciences; Mesenchymal stem cells; Mesenchymal Stromal Cells - cytology; Mesenchyme; mRNA; multidisciplinary; Peripheral blood; Peripheral Blood Stem Cells - cytology; Proteins; Science; SOX9 Transcription Factor - genetics; SOX9 Transcription Factor - metabolism; Stem cell transplantation; Stem cells; Swine; Tissue engineering; Tissue Engineering - methods; Tissue Scaffolds
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep36400

As a cell source with large quantity and easy access, peripheral blood mesenchymal stem cells (PBMSCs) were isolated and seeded in porcine demineralized cancellous bone (DCB) scaffolds, cultured in chondrogenic medium and evaluated for in vitro chondrogenesis. Bone marrow MSCs (BMMSCs) and articular cartilage chondrocytes (ACCs) underwent the same process as controls. The morphology, viability and proliferation of PBMSCs in DCB scaffolds were similar to those of BMMSCs and ACCs. PBMSCs and BMMSCs showed similar chondrogenesis potential with consistent production of COL 2 and SOX 9 protein and increased COL 2 and AGC mRNA expressions at week 3 but the COL 2 protein production was still less than that of ACCs. Minimal increase of hypertrophic markers was found in all groups. Relatively higher ALP and lower COL 10 mRNA expressions were found in both MSCs groups at week 3 than that in ACCs, whereas no significant difference of COL 1 and SOX 9 mRNA and MMP 13 protein was found among all groups. To conclude, PBMSCs shared similar proliferation and chondrogenic potential with BMMSCs in DCB scaffolds and could be an alternative to BMMSCs for cartilage tissue engineering. Further optimization of chondrogenesis system is needed regardless of the promising results.