Inhibitory effect of Ruta graveolens L. on oxidative damage, inflammation and aortic pathology in hypercholesteromic rats

• Published in: Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie Vol. 63; no. 3; pp. 285 - 290
• Main Authors: Ratheesh, M., Shyni, G.L., Sindhu, G., Helen, A.
• Format: Journal Article
• Language: English
• Published: Munich Elsevier GmbH 01.03.2011; Elsevier
• Subjects: Animals; Anti-inflammatory; Antioxidant; antioxidant activity; Antioxidants - isolation & purification; Antioxidants - therapeutic use; aorta; Aorta - drug effects; Aorta - pathology; Aortic; Atherosclerosis; Atherosclerosis (general aspects, experimental research); Atherosclerosis - pathology; Atherosclerosis - prevention & control; Biological and medical sciences; Blood and lymphatic vessels; blood serum; Cardiology. Vascular system; Cells, Cultured; cholesterol; Cyclooxygenase; Disease Models, Animal; glutathione; heart; histopathology; Hypercholesterolemia - complications; Hypercholesterolemia - drug therapy; Hypercholesterolemia - pathology; inflammation; Investigative techniques, diagnostic techniques (general aspects); leukocyte count; Leukocytes, Mononuclear - enzymology; Lipoxygenase; Lipoxygenase - metabolism; liver; Male; Medical sciences; monocytes; myeloperoxidase; oxidative stress; Oxidative Stress - drug effects; Pathology; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Plant Extracts - isolation & purification; Plant Extracts - therapeutic use; Prostaglandin-Endoperoxide Synthases - metabolism; Rats; Rats, Sprague-Dawley; Ruta - chemistry; Ruta graveolens; thiobarbituric acid-reactive substances; Pathology; Cyclooxygenase; Aortic; Atherosclerosis; Antioxidant; Lipoxygenase; Anti-inflammatory; Prostaglandin-endoperoxide synthase; Oxidative stress; Rat; Cardiovascular disease; Vascular disease; Blood vessel; Aorta; Enzyme; Rodentia; Antiinflammatory agent; Inflammation; Artery; Vertebrata; Anatomic pathology; Mammalia; Animal; Circulatory system; Oxidoreductases
• ISSN: 0940-2993; 1618-1433
• DOI: 10.1016/j.etp.2010.01.007

The purpose of the study was to investigate the efficacy of methanolic extract of Ruta graveolens L. in reducing oxidative damage, inflammation and aortic pathology in hypercholesteremic rats. For the study rats were divided into three groups – control group, hypercholesteremic group and treatment group (20 mg MER/kg/d orally) – and were fed for 90 days. Serum total cholesterol, LDL-C, total WBC count, CRP level, TBARS, atherogenic index, activities of COX, 15 LOX in monocyte and serum myeloperoxidase were increased in cholesterol fed rats. Activities of antioxidant enzymes and the concentration reduced glutathione in liver and heart tissue and serum HDL-C were decreased in cholesterol fed rats. The results showed that level of total cholesterol, LDL-C, atherogenic index was decreased and HDL-C was increased in MER treated rats . Activities of antioxidant enzymes were found to be increased and the activity of MPO, COX and 15 LOX were decreased on supplementation with MER. Concentration of TBARS and total WBC count were decreased and GSH was increased on supplementation with MER. Histopathology of aorta of cholesterol fed rat showed marked alterations whereas the aorta of MER administrated rat showed no significant changes. These results suggested that MER reduces oxidative stress, inflammation and aortic pathology in hypercholesteremic rats. Thus the plant may therefore be useful for therapeutic treatment of clinical conditions associated atherosclerosis.