Antioxidant acitivity of low molecular weight hyaluronic acid

• Published in: Food and chemical toxicology Vol. 49; no. 10; pp. 2670 - 2675
• Main Authors: Ke, Chunlin, Sun, Lanping, Qiao, Deliang, Wang, Di, Zeng, Xiaoxiong
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.10.2011; Elsevier
• Subjects: Animals; Antioxidant activity; Antioxidants - pharmacology; Biological and medical sciences; Biphenyl Compounds - metabolism; catalase; Catalase - blood; cyclophosphamide; free radical scavengers; Free Radical Scavengers - pharmacology; glutathione peroxidase; Glutathione Peroxidase - blood; Hyaluronic acid; Hyaluronic Acid - pharmacology; hydroxyl radicals; Immunocompromised Host; Immunosuppressed mice; lipid peroxidation; Low molecular weight; Male; Malondialdehyde - blood; Medical sciences; Mice; molecular weight; Oxidative Stress - drug effects; Picrates - metabolism; Random Allocation; superoxide anion; superoxide dismutase; Superoxide Dismutase - blood; Toxicology; Hyaluronic acid; Antioxidant activity; Low molecular weight; Immunosuppressed mice; Rodentia; Antioxidant; Biological activity; Vertebrata; Immunosuppression; Mammalia; Mouse; Healing agent; Animal; Antirheumatic agent; Molecular mass
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2011.07.020

► Hyaluronic acid was partially degraded by sulfate radical to afford low molecular weight hyaluronic acid (LMWHA). ► LMWHA showed strong inhibition of lipid peroxidation and hydroxyl radical scavenging activity in vitro. ► LMWHA was able to overcome cyclophosphamide-induced immunosuppression in mice. ► LMWHA was able to significantly raise the antioxidant enzymes activities in immunosuppressed mice. Two polysaccharides, low molecular weight hyaluronic acid-1 (LMWHA-1) and LMWHA-2, with their molecular weight of 1.45×105 and 4.52×104Da, respectively, were prepared from high molecular weight hyaluronic acid (HA,1.05×106Da). LMWHA-1, LMWHA-2 and HA were studied for their antioxidant activities. In vitro antioxidant assay, LMWHA showed strong inhibition of lipid peroxidation and scavenging activities of hydroxyl radical, moderate 1,1-diphenyl-2-picryldydrazyl radical and superoxide anion scavenging activity. In addition, the LMWHA-1 exhibited much stronger antioxidant activity than LMWHA-2 and HA. For antioxidant testing in vivo, LMWHA-1, LMWHA-2 and HA were orally administrated over a period of 7days in a cyclophosphamide(CY) induced immunosuppressed mice model. As results, administration of LMWHA was able to overcome CY-induced immunosuppression and significantly raised the activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and total antioxidant capacity (TAOC) in immunosuppressed mice. The results showed that the LMWHA, possessing pronounced free radical scavenging and antioxidant activities.