Effect of Same-dose Single or Dual Field Irradiation on Damage to Miniature Pig Parotid Glands

• Published in: International journal of oral science Vol. 1; no. 1; pp. 16 - 25
• Main Authors: Yan, Xing, Hai, Bo, Shan, Zhao‐chen, Zheng, Chang‐yu, Zhang, Chun‐mei, Wang, Song‐lin
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.03.2009; Springer Nature B.V; Beijing Friendship Hospital, Capital Medical University, Beijing, China%Beijing Friendship Hospital, Capital Medical University, Beijing, China%Salivary Gland Disease Center and the Molecular Laboratory for Gene Therapy, Capital Medical University School of Stomatology, Beijing, China%Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, NIH, DHHS, Bethesda,MD, USA%Salivary Gland Disease Center and the Molecular Laboratory for Gene Therapy, Capital Medical University School of Stomatology, Beijing, China; Department of Biochemistry and Molecular Biology, Capital Medical University, Beijing, China; Salivary Gland Disease Center and the Molecular Laboratory for Gene Therapy, Capital Medical University School of Stomatology, Beijing, China; Nature Publishing Group
• Subjects: Amylases - analysis; Amylases - blood; Amylases - radiation effects; Animals; Blood Platelets - radiation effects; Calcium - analysis; Calcium - radiation effects; Dentistry; Erythrocyte Count; Erythrocytes - radiation effects; L-Lactate Dehydrogenase - blood; L-Lactate Dehydrogenase - radiation effects; Leukocyte Count; Leukocytes - radiation effects; Male; Medicine; Models, Animal; Oral and Maxillofacial Surgery; Organ Size - radiation effects; Original Scientific; original-scientific-article; Orthopedics; Parotid Gland - pathology; Parotid Gland - radiation effects; Potassium - analysis; Potassium - radiation effects; Radiation Dosage; Random Allocation; Saliva - chemistry; Saliva - radiation effects; Secretory Rate - radiation effects; Surgical Orthopedics; Swine; Swine, Miniature; Time Factors; 口腔疾病; 口腔科学; 药物治疗; irradiation damage; miniature pig; parotid gland
• ISSN: 1674-2818; 2049-3169
• DOI: 10.4248/ijos.08001

Aim To evaluate the effect of single or dual field irra- diation （IR） with the same dose on damage to miniature pig parotid glands. Methodology Sixteen miniature pigs were divided into two IR groups （n=6） and a control group （n=4）. The irradi- ation groups were subjected to 20 Gy X-radiation to one parotid gland using single-field or dual-field modality by linear accelerator. The dose-volume distributions between two IR groups were compared. Saliva from parotid glands and blood were collected at 0, 4, 8 and 16 weeks after irradiation. Parotid glands were removed at 16 weeks to evaluate tissue morphology. Results The irradiation dose volume distributions were significantly different between single and dual field irradi- ation groups （t---4.177, P=0.002）, although dose volume histogramin （DVH） indicated the equal maximal dose in parotid glands. Saliva flow rates from IR side decreased dramatically at all time points in IR groups, especially in dual field irradiation group. The radiation caused changes of white blood cell count in blood, lactate dehydrogenase and amylase in serum, calcium, potassium and amylase in saliva. Morphologically, more severe radiation damage was found in irradiated parotid glands from dual field irradi- ation group than that from single field irradiation group. Conclusion Data from this large animal model demons- trated that the radiation damage from the dual field irradiation was more severe than that of the single field irradiation at the same dose, suggesting that dose-volume distribution is an important factor in evaluation of the radiobiology of parotid glands.