Chemical and Physical Characteristics of Doxorubicin Hydrochloride Drug-Doped Salmon DNA Thin Films

• Published in: Scientific reports Vol. 5; no. 1; p. 12722
• Main Authors: Gnapareddy, Bramaramba, Reddy Dugasani, Sreekantha, Ha, Taewoo, Paulson, Bjorn, Hwang, Taehyun, Kim, Taesung, Hoon Kim, Jae, Oh, Kyunghwan, Park, Sung Ha
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 31.07.2015; Nature Publishing Group
• Subjects: 631/61/54/152; 639/301/54/992; 639/925/350/59; Animals; Binding sites; Deoxyribonucleic acid; DNA; DNA - chemistry; DNA - metabolism; Doxorubicin; Doxorubicin - chemistry; Drug Carriers; Fourier transforms; Humanities and Social Sciences; Infrared spectroscopy; Luminescence; Luminescent Measurements; multidisciplinary; Nucleosides; Photons; Physical characteristics; Quartz; Salmon - genetics; Science; Spectrophotometry, Ultraviolet; Spectroscopy, Fourier Transform Infrared; Spectrum analysis; Thin films
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep12722

Double-stranded salmon DNA (SDNA) was doped with doxorubicin hydrochloride drug molecules (DOX) to determine the binding between DOX and SDNA and DOX optimum doping concentration in SDNA. SDNA thin films were prepared with various concentrations of DOX by drop-casting on oxygen plasma treated glass and quartz substrates. Fourier transform infrared (FTIR) spectroscopy was employed to investigate the binding sites for DOX in SDNA and electrical and photoluminescence (PL) analyses were used to determine the optimum doping concentration of DOX. The FTIR spectra showed that up to a concentration of 30 μM of DOX, there was a tendency for binding with a periodic orientation via intercalation between nucleosides. The current and PL intensity increased as the DOX concentration increased up to 30 μM and then as the concentration of DOX further increased, we observed a decrease in current as well as PL quenching. Finally, the optical band gap and second band onset of the transmittance spectra were analyzed to further verify the DOX binding and optimum doping concentration into SDNA thin films as a function of the DOX concentration.