Investigation of the Origin of Extracellular RNA in Human Cell Culture

:  We have used the human ECV 304 cell line to study the origin and fate of extracellular RNA (exRNA) in cell culture. Quantification of different extracellular RNA species using reverse transcription followed by quantitative PCR revealed a prevalent fraction of ribosomal RNAs. Comparison of intrace...

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Bibliographic Details
Published inAnnals of the New York Academy of Sciences Vol. 1075; no. 1; pp. 50 - 56
Main Authors BÖTTCHER, KATRIN, WENZEL, ALEXANDER, WARNECKE, JENS M.
Format Journal Article
LanguageEnglish
Published Malden, USA Blackwell Publishing Inc 01.09.2006
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Summary::  We have used the human ECV 304 cell line to study the origin and fate of extracellular RNA (exRNA) in cell culture. Quantification of different extracellular RNA species using reverse transcription followed by quantitative PCR revealed a prevalent fraction of ribosomal RNAs. Comparison of intracellular and extracellular ribosomal RNA copy numbers allowed the calculation of the number of destroyed cells that would result in the corresponding number of extracellular rRNAs. Interestingly, this number was comparable to the amount of destroyed cells as determined by the measurement of extracellular lactate dehydrogenase activity.
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ISSN:0077-8923
1749-6632
DOI:10.1196/annals.1368.006