Identification and characterization of an insular auditory field in mice

• Published in: The European journal of neuroscience Vol. 34; no. 12; pp. 1944 - 1952
• Main Authors: Sawatari, Hiroyuki, Tanaka, Yoshihide, Takemoto, Makoto, Nishimura, Masataka, Hasegawa, Kayoko, Saitoh, Kazuya, Song, Wen-Jie
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.12.2011
• Subjects: Acoustic Stimulation - methods; Animals; Auditory Cortex - anatomy & histology; Auditory Cortex - physiology; Brain Mapping - methods; Computed tomography; Cortex (auditory); Cortex (insular); cortical parcellation; Fluorescent Dyes - metabolism; Humans; Mice; Mice, Inbred C57BL; Nervous system; Neuroimaging; optical imaging; response dynamics; tonotopy
• ISSN: 0953-816X; 1460-9568
• DOI: 10.1111/j.1460-9568.2011.07926.x

We used voltage‐sensitive‐dye‐based imaging techniques to identify and characterize the insular auditory field (IAF) in mice. Previous research has identified five auditory fields in the mouse auditory cortex, including the primary field and the anterior auditory field. This study confirmed the existence of the primary field and anterior auditory field by examining the tonotopy in each field. Further, we identified a previously unreported IAF located rostral to known auditory fields. Pure tone evoked responses in the IAF exhibited the shortest latency among all auditory fields at lower frequencies. A rostroventral to dorsocaudal frequency gradient was consistently observed in the IAF in all animals examined. Neither the response amplitude nor the response duration changed with frequency in the IAF, but the area of activation exhibited a significant increase with decreasing tone frequency. Taken together, the current results indicate the existence of an IAF in mice, with characteristics suggesting a role in the rapid detection of lower frequency components of incoming sound. We used voltage‐sensitive‐dye‐based imaging techniques to identify and characterize the insular auditory field (IAF) in mice. Previous research has identified five auditory fields in the mouse auditory cortex, including the primary field and the anterior auditory field. This study confirmed the existence of the primary field and anterior auditory field by examining the tonotopy in each field.