Liposome-mediated DNA uptake by sperm cells

To investigate the potential use of sperm cells as vectors to transfer exogenous DNA via the fertilization of oocytes into the germ line of mice, we have used liposomes to transfect DNA into the sperm head. Although the DNA transfer into sperm mediated by liposomes was very efficient and no obvious...

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Bibliographic Details
Published inMolecular reproduction and development Vol. 30; no. 3; p. 194
Main Authors Bachiller, D, Schellander, K, Peli, J, Rüther, U
Format Journal Article
LanguageEnglish
Published United States 01.11.1991
Subjects
Animals
DNA - metabolism
Fertilization in Vitro
Fluorescent Antibody Technique
Gene Expression
Genetic Engineering
Liposomes
Male
Mice
Mice, Transgenic
Phosphatidylethanolamines
Spermatozoa - metabolism
Transfection
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Summary:To investigate the potential use of sperm cells as vectors to transfer exogenous DNA via the fertilization of oocytes into the germ line of mice, we have used liposomes to transfect DNA into the sperm head. Although the DNA transfer into sperm mediated by liposomes was very efficient and no obvious reduction in the fertilization frequency of oocytes could be detected, we were unable to generate transgenic mice by this method.
ISSN:1040-452X
DOI:10.1002/mrd.1080300305