Prevalence, antimicrobial resistance, virulence gene profile and molecular typing of Campylobacter species isolated from poultry meat samples

• Published in: Veterinary medicine and science Vol. 8; no. 6; pp. 2482 - 2493
• Main Authors: Hadiyan, Maryam, Momtaz, Hassan, Shakerian, Amir
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.11.2022; John Wiley and Sons Inc; Wiley
• Subjects: Ampicillin; Animals; Anti-Bacterial Agents - pharmacology; Antibiotic resistance; Antimicrobial agents; Antimicrobial resistance; Bacteria; Campylobacter; Campylobacter coli; Campylobacter jejuni; Chloramphenicol; Ciprofloxacin; Drug resistance; Drug Resistance, Bacterial - genetics; Erythromycin; FlaA protein; Food contamination & poisoning; Gastrointestinal diseases; Genes; Guillain-Barre syndrome; Humans; Infections; Meat; Meat - microbiology; molecular typing; Molecular Typing - veterinary; Original; Polymerase chain reaction; Population studies; POULTRY; Poultry - genetics; Poultry - microbiology; Prevalence; raw poultry meat; Typing; Virulence; Virulence - genetics; Virulence factors; Virulence Factors - genetics; United Kingdom > UK; Iran; United States > US; Germany; raw poultry meat; antimicrobial resistance; virulence factors; Campylobacter coli; molecular typing; Campylobacter jejuni
• ISSN: 2053-1095; 2053-1095
• DOI: 10.1002/vms3.944

Background Campylobacter jejuni and Campylobacter coli are the most significant Campylobacter species responsible for severe gastrointestinal disorders. Raw poultry meat is considered a source of Campylobacter transmission to the human population. Objectives The present study was aimed to assess the prevalence rate, antibiotic resistance properties, virulence characters and molecular typing of C. jejuni and C. coli strains isolated from raw poultry meat samples. Methods Three hundred and eighty raw poultry meat samples were collected and analysed for the presence of Campylobacter spp. using the microbial culture. Species identification was done using the Polymerase Chain Reaction. Disk diffusion was developed to assess the antimicrobial resistance pattern of isolates. The distribution of virulence and antimicrobial resistance genes was determined by PCR. Enterobacterial Repetitive Intergenic Consensus‐PCR was used for molecular typing. Results Campylobacter species were isolated from 6.25% of examined samples. C. jejuni and C. coli contamination rates were found to be 57.44% and 48.14%, respectively. C. jejuni strains harboured the highest resistance rate against serythromycin (42.59%), ampicillin (38.88%), ciprofloxacin (33.33%), chloramphenicol (31.48%) and tetracycline (31.48%). C. coli isolates harboured the highest resistance rate against ampicillin (73.07%), ciprofloxacin (73.07%), erythromycin (65.38%) and chloramphenicol (50%). AadE1 (44.44%), blaOXA‐61 (42.59%) and tet(O) (35.18%) were the most commonly detected resistance genes in C. jejuni and cmeB (34.61%) and blaOXA‐61 (34.61%) were the most commonly detected among C. coli strains. The most frequent virulence factors among the C. jejuni isolates were flaA (100%), ciaB (100%), racR (83.33%), dnaJ (81.48%), cdtB (81.48%), cdtC (79.62%) and cadF (74.07%). The most frequent virulence factors among the C. coli isolates were flaA (100%), ciaB (100%), pldA (65.38%) and cadF (61.53%). Conclusions The majority of C. jejuni and C. coli strains had more than 80% similarities in their ERIC‐PCR pattern, which may show their common source of transmission. The role of goose and quebec meat samples as reservoirs of virulent and antimicrobial resistant Campylobacter spp. was determined. Chicken and Turkey meat samples harboured the highest contamination rates. C. jejuni and C. coli isolates harboured a high resistance rate against some antibiotics, which was accompanied by the high distribution of most antimicrobial resistance‐encoding genes.