Expression, purification and crystallization of an indole prenyltransferase from Aspergillus fumigatus

• Published in: Acta crystallographica. Section F, Structural biology and crystallization communications Vol. 68; no. 3; pp. 355 - 358
• Main Authors: Chen, Jing, Morita, Hiroyuki, Kato, Ryohei, Noguchi, Hiroshi, Sugio, Shigetoshi, Abe, Ikuro
• Format: Journal Article
• Language: English
• Published: 5 Abbey Square, Chester, Cheshire CH1 2HU, England International Union of Crystallography 01.03.2012
• Subjects: alkaloids; Aspergillus fumigatus; Aspergillus fumigatus - enzymology; Crystallization; Crystallization Communications; Crystallography, X-Ray; Dimethylallyltranstransferase - chemistry; Dimethylallyltranstransferase - genetics; Dimethylallyltranstransferase - isolation & purification; Escherichia coli; Gene Expression; prenyltransferases; tryptophan
• ISSN: 1744-3091; 1744-3091
• DOI: 10.1107/S1744309112004964

CdpNPT from Aspergillus fumigatus is a dimethylallyltryptophan synthase/indole prenyltransferase that catalyzes reverse prenylation at position N1 of tryptophan‐containing cyclic dipeptides. Residues 38–440 of CdpNPT were expressed in Escherichia coli and crystallized using the sitting‐drop vapour‐diffusion and microseeding techniques. The crystals belonged to space group P212121, with unit‐cell parameters a = 84.4, b = 157.1, c = 161.8 Å, α = β = γ = 90.0°.