The structure of a stable intermediate in the A ↔ B DNA helix transition

The DNA dodecamer CATGGGCCCATG in a crystal structure of resolution 1.3 Å has a conformation intermediate between A and B DNA. This trapping of a stable intermediate suggests that the A and B DNA families are not discrete, as previously believed. The structure supports a base-centered rather than a...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 97; no. 5; pp. 2035 - 2039
Main Authors Ng, H L, Kopka, M L, Dickerson, R E
Format Journal Article
LanguageEnglish
Published United States National Acad Sciences 29.02.2000
The National Academy of Sciences
Subjects
Amino Acid Sequence
Base Sequence
Biological Sciences
Crystallography, X-Ray
DNA - chemistry
Molecular Sequence Data
Nucleic Acid Conformation
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Summary:The DNA dodecamer CATGGGCCCATG in a crystal structure of resolution 1.3 Å has a conformation intermediate between A and B DNA. This trapping of a stable intermediate suggests that the A and B DNA families are not discrete, as previously believed. The structure supports a base-centered rather than a backbone-centered mechanism for the A ↔ B transition mediated by guanine tracts. Interconversion between A and B DNA provides another means for regulating protein–DNA recognition.
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Contributed by Richard E. Dickerson
To whom reprint requests should be addressed. E-mail: red@mbi.ucla.edu.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.040571197