Zearalenone Induces Blood-Testis Barrier Damage through Endoplasmic Reticulum Stress-Mediated Paraptosis of Sertoli Cells in Goats

• Published in: International journal of molecular sciences Vol. 25; no. 1; p. 553
• Main Authors: Liu, Tengfei, Liu, Gengchen, Xu, Yinghuan, Huang, Yuqi, Zhang, Yunxuan, Wu, Yongjie, Xu, Yongping
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 31.12.2023; MDPI
• Subjects: Animals; Apoptosis; Autophagy; blood-testis barrier; Cell death; Endoplasmic reticulum; endoplasmic reticulum stress; Estrogens; Fertility; Food contamination; goat; Goats; Infertility; Localization; Male reproductive system; Medical research; Medicine, Experimental; Morphology; Motility; paraptosis; Proteins; Sertoli cells; Spermatogenesis; Testes; Toxicity; zearalenone; Massachusetts; Japan; China; Missouri; zearalenone; goat; paraptosis; blood-testis barrier; Sertoli cells; endoplasmic reticulum stress
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms25010553

Zearalenone (ZEA) is present worldwide as a serious contaminant of food and feed and causes male reproductive toxicity. The implication of paraptosis, which is a nonclassical paradigm of cell death, is unclear in ZEA-induced male reproductive disorders. In this study, the toxic effects of ZEA on the blood-testis barrier (BTB) and the related mechanisms of paraptosis were detected in goats. ZEA exposure, in vivo, caused a significant decrease in spermatozoon quality, the destruction of seminiferous tubules, and damage to the BTB integrity. Furthermore, ZEA exposure to Sertoli cells (SCs) in vitro showed similar dysfunction in structure and barrier function. Importantly, the formation of massive cytoplasmic vacuoles in ZEA-treated SCs corresponded to the highly swollen and dilative endoplasmic reticulum (ER), and paraptosis inhibition significantly alleviated ZEA-induced SC death and vacuolization, which indicated the important contribution of paraptosis in ZEA-induced BTB damage. Meanwhile, the expression of ER stress marker proteins was increased after ZEA treatment but decreased under the inhibition of paraptosis. The vacuole formation and SC death, induced by ZEA, were remarkably blocked by ER stress inhibition. In conclusion, these results facilitate the exploration of the mechanisms of the SC paraptosis involved in ZEA-induced BTB damage in goats.