siRNA-mediated knockdown of the melanocortin 2 receptor accessory protein 2 (MRAP2) gene confers resistance to methylmercury on HEK293 cells

Methylmercury is a well-known environmental pollutant that causes serious disorders of the central nervous system as well as a range of other symptoms. We employed small interfering RNA (siRNA) to search for factors in ligand-dependent signal transduction pathways that may be involved in the develop...

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Published inJournal of toxicological sciences Vol. 35; no. 6; pp. 947 - 950
Main Authors Hwang, Gi-Wook, Oh, Seong-Eun, Takahashi, Tsutomu, Lee, Jin-Yong, Naganuma, Akira
Format Journal Article
LanguageEnglish
Published Japan The Japanese Society of Toxicology 01.12.2010
Subjects
Carrier Proteins - antagonists & inhibitors
Carrier Proteins - genetics
Drug Resistance - genetics
Environmental Pollutants - toxicity
HEK293 Cells
Humans
Ligands
Melanocortin
Methylmercury
Methylmercury Compounds - toxicity
MRAP2
Resistance
RNA, Small Interfering - genetics
Signal Transduction - drug effects
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Summary:Methylmercury is a well-known environmental pollutant that causes serious disorders of the central nervous system as well as a range of other symptoms. We employed small interfering RNA (siRNA) to search for factors in ligand-dependent signal transduction pathways that may be involved in the development of methylmercury toxicity. Melanocortin 2 receptor accessory protein 2 (MRAP2) is involved in the melanocortin pathway. Using siRNA, we found that decreased expression of MRAP2 conferred strong methylmercury resistance in HEK293 cells.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0388-1350
1880-3989
1880-3989
DOI:10.2131/jts.35.947