Innate immunity gene single nucleotide polymorphisms and otitis media

• Published in: International journal of pediatric otorhinolaryngology Vol. 76; no. 7; pp. 976 - 979
• Main Authors: Carroll, Sarah R., Zald, Philip B., Soler, Zachary M., Milczuk, Henry A., Trune, Dennis R., MacArthur, Carol J.
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 01.07.2012
• Subjects: Adolescent; Apnea; Case-Control Studies; CD14 antigen; Child; Child, Preschool; Children; Female; Genetic Predisposition to Disease; genomics; Genotyping; Humans; Hypersensitivity; Immune system; Immunity; Immunity, Innate - genetics; Infant; Infection; Innate immune system; Lipopolysaccharide Receptors - genetics; Male; Otitis media; Otitis Media - genetics; Otitis Media - immunology; Otolaryngology; Pediatrics; Polymerase chain reaction; Polymorphism, Single Nucleotide; Single nucleotide polymorphisms; Single-nucleotide polymorphism; Sleep; Sleep disorders; Smoke; Surgery; TLR2 protein; TLR4 protein; TLR9 protein; Toll-like receptors; Toll-Like Receptors - genetics; Toll-like receptors; Otitis media; Innate immune system; Single nucleotide polymorphisms
• ISSN: 0165-5876; 1872-8464; 1872-8464
• DOI: 10.1016/j.ijporl.2012.03.011

Toll-like receptors (TLR) activate the innate immune system. Single nucleotide polymorphisms (SNPs) in TLR genes are linked to increased susceptibility to infections. TLR4-deficient mice have increased incidence and duration of otitis media. We hypothesize that SNPs in TLR genes are more common in otitis-prone children than in children without a history of otitis media. Cases (n=70) included children undergoing surgery for otitis media. Control subjects (n=70) included children undergoing surgery for non-otologic indication. Genomic DNA was extracted from blood samples. RT-PCR genotyping was performed for TLR2 (rs5743708), TLR4 (rs4986790 and rs4986791), TLR9 (rs5743836 & rs187084), and CD14 (rs2569190). There were no significant differences between the groups in family history, day care, smoke exposure, allergies or prevalence of the SNPs. The most common pre-op diagnosis in control subjects was obstructive sleep apnea (OSA). TLR2, TLR4, TLR9 and CD14 gene SNPs were not more prevalent in otitis-prone children.