Expression of resistance gene analogs in woodland strawberry (Fragaria vesca) during infection with Phytophthora cactorum

• Published in: Molecular genetics and genomics : MGG Vol. 291; no. 5; pp. 1967 - 1978
• Main Authors: Chen, Xiao-Ren, Brurberg, May Bente, Elameen, Abdelhameed, Klemsdal, Sonja Sletner, Martinussen, Inger
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.10.2016; Springer Nature B.V
• Subjects: Animal Genetics and Genomics; Binding sites; Biochemistry; Biomedical and Life Sciences; Cloning, Molecular; Disease Resistance; Flowers & plants; Fragaria; Fragaria - genetics; Fragaria - parasitology; Fragaria vesca; Gene Expression Profiling - methods; Gene Expression Regulation, Plant; Genes; Genomes; Genomics; Human Genetics; Infections; Kinases; Life Sciences; Microbial Genetics and Genomics; Oomycetes; Original; Original Article; Pathogens; Phytophthora - pathogenicity; Phytophthora cactorum; Plant Diseases - genetics; Plant Diseases - parasitology; Plant Genetics and Genomics; Plant Proteins - genetics; Sequence Analysis, DNA; NBS-LRR genes; Crown rot disease; Resistance gene analogs; Strawberry; Phytophthora cactorum
• ISSN: 1617-4615; 1617-4623
• DOI: 10.1007/s00438-016-1232-x

Important losses in strawberry production are often caused by the oomycete Phytophthora cactorum , the causal agent of crown rot. However, very limited studies at molecular levels exist of the mechanisms related to strawberry resistance against this pathogen. To begin to rectify this situation, a PCR-based approach (NBS profiling) was used to isolate strawberry resistance gene analogs (RGAs) with altered expression in response to P. cactorum during a time course (2, 4, 6, 24, 48, 96 and 192 h post-infection). Twenty-three distinct RGA fragments of the NB-LRR type were identified from a resistance genotype (Bukammen) of the wild species Fragaria vesca . The gene transcriptional profiles after infection showed that the response of most RGAs was quicker and stronger in the resistance genotype (Bukammen) than in the susceptible one (FDP821) during the early infection stage. The transcriptional patterns of one RGA (RGA109) were further monitored and compared during the P. cactorum infection of two pairs of resistant and susceptible genotype combinations (Bukammen/FDP821 and FDR1218/1603). The 5′ end sequence was cloned, and its putative protein was characteristic of NBS-LRR R protein. Our results yielded a first insight into the strawberry RGAs responding to P. cactorum infection at molecular level.