Genome-Wide Identification and Expression Analysis of the Stearoyl-Acyl Carrier Protein Δ9 Desaturase Gene Family under Abiotic Stress in Barley

• Published in: International journal of molecular sciences Vol. 25; no. 1; p. 113
• Main Authors: Ding, Mingyu, Zhou, Danni, Ye, Yichen, Wen, Shuting, Zhang, Xian, Tian, Quanxiang, Zhang, Xiaoqin, Mou, Wangshu, Dang, Cong, Fang, Yunxia, Xue, Dawei
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 21.12.2023; MDPI
• Subjects: Abiotic stress; Abscisic acid; Amino acids; Barley; Bioinformatics; Cotton; evolution; Fatty acids; Genes; Genomes; Genomics; Oilseeds; Phylogenetics; Phylogeny; Physiological aspects; Proteins; Rape plants; SAD; Seeds; Unsaturated fatty acids; unsaturated fatty acids (UFAs); Wheat; China; unsaturated fatty acids (UFAs); barley; evolution; SAD; abiotic stress
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms25010113

Stearoyl-acyl carrier protein (ACP) Δ9 desaturase (SAD) is a critical fatty acid dehydrogenase in plants, playing a prominent role in regulating the synthesis of unsaturated fatty acids (UFAs) and having a significant impact on plant growth and development. In this study, we conducted a comprehensive genomic analysis of the SAD family in barley ( L.), identifying 14 HvSADs with the FA_desaturase_2 domain, which were divided into four subgroups based on sequence composition and phylogenetic analysis, with members of the same subgroup possessing similar genes and motif structures. Gene replication analysis suggested that tandem and segmental duplication may be the major reasons for the expansion of the family in barley. The promoters of contained various -regulatory elements (CREs) related to light, abscisic acid (ABA), and methyl jasmonate (MeJA). In addition, expression analysis indicated that exhibit multiple tissue expression patterns in barley as well as different response characteristics under three abiotic stresses: salt, drought, and cold. Briefly, this evolutionary and expression analysis of provides insight into the biological functions of barley, supporting a comprehensive analysis of the regulatory mechanisms of oil biosynthesis and metabolism in plants under abiotic stress.