Effects of taxifolin on the activity of angiotensin-converting enzyme and reactive oxygen and nitrogen species in the aorta of aging rats and rats treated with the nitric oxide synthase inhibitor and dexamethasone

• Published in: AGE Vol. 35; no. 6; pp. 2089 - 2097
• Main Authors: Arutyunyan, Tamara V., Korystova, Antonina F., Kublik, Ludmila N., Levitman, Maria Kh, Shaposhnikova, Vera V., Korystov, Yuri N.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.12.2013; Springer Nature B.V
• Subjects: Age; Aging; Aging - drug effects; Aging - metabolism; Animals; Anti-Inflammatory Agents, Non-Steroidal - pharmacology; Aorta, Thoracic - drug effects; Aorta, Thoracic - growth & development; Aorta, Thoracic - metabolism; Atherosclerosis; Atherosclerosis - metabolism; Atherosclerosis - pathology; Atherosclerosis - prevention & control; Biomedical and Life Sciences; Biophysics; Cell Biology; Cell division; Coronary vessels; Dexamethasone - pharmacology; Disease Models, Animal; Drinking water; Endothelium; Endothelium, Vascular - drug effects; Enzyme Inhibitors; Enzymes; Flavonoids; Geriatrics/Gerontology; Glucocorticoids - pharmacology; Laboratory animals; Life Sciences; Lipids; Male; Molecular Medicine; NG-Nitroarginine Methyl Ester - pharmacology; Nitric oxide; Nitric Oxide Synthase - pharmacology; Nitrogen; Nitrogen - metabolism; Oxidation; Oxidative stress; Peptidyl-Dipeptidase A - drug effects; Peptidyl-Dipeptidase A - metabolism; Quercetin - analogs & derivatives; Quercetin - pharmacology; Rats; Rats, Wistar; Reactive Oxygen Species - metabolism; Rodents; Thorax; L-NAME; Dexamethasone; Aging; ROS/RNS; Aorta; Taxifolin; Angiotensin-converting enzyme
• ISSN: 0161-9152; 2509-2715; 1574-4647; 2509-2723
• DOI: 10.1007/s11357-012-9497-4

The action of taxifolin on the angiotensin-converting enzyme (ACE) and the formation of reactive oxygen and nitrogen species (ROS/RNS) in the aorta of aging rats and rats treated with nitric oxide synthase inhibitor ( N ω -nitro- l -arginine methyl ester (L-NAME)) or dexamethasone have been studied. The ACE activity in aorta sections was determined by measuring the hydrolysis of hippuryl- l -histidyl- l -leucine, and the ROS/RNS production was measured by oxidation of dichlorodihydrofluorescein. It was shown that taxifolin at a dose of 30–100 μg/kg/day decreases the ACE activity in the aorta of aging rats and of rats treated with L-NAME or dexamethasone to the level of the ACE activity in young control rats. Taxifolin (100 μg/kg/day) was found to also reduce the amount of ROS/RNS in the aorta that increased as a result of L-NAME intake. L-NAME treatment increases the contribution of 5-lipoxygenase and NADPH oxidase to ROS/RNS production in the aorta, while taxifolin (100 μg/kg/day) decreases the contribution of these enzymes to the normal level.