RNAi-mediated down-regulation of SHATTERPROOF gene in transgenic oilseed rape

• Published in: 3 Biotech Vol. 5; no. 3; pp. 271 - 277
• Main Authors: Kord, Hadis, Shakib, Ali Mohammad, Daneshvar, Mohammad Hossein, Azadi, Pejman, Bayat, Vahid, Mashayekhi, Mohsen, Zarea, Mahboobeh, Seifi, Alireza, Ahmad-Raji, Mana
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.06.2015; Springer Nature B.V
• Subjects: Agricultural biotechnology; Agricultural production; Agriculture; Bioinformatics; Biomaterials; Biotechnology; Brassica; Cancer Research; Chemistry; Chemistry and Materials Science; Genes; Genetic engineering; Oilseeds; Original; Original Article; Rape plants; Seeds; Stem Cells; Transgenic plants; Iran; Oilseed rape; Gene silencing; RNAi; Pod shattering; gene; BnSHP gene
• ISSN: 2190-572X; 2190-5738
• DOI: 10.1007/s13205-014-0226-9

Oilseed rape is one of the important oil plants. Pod shattering is one of the problems in oilseed rape production especially in regions with dry conditions. One of the important genes in Brassica pod opening is SHATTERPROOF1 ( SHP1 ). Down-regulation of BnSHP1 expression by RNAi can increase resistance to pod shattering. A 470 bp of the BnSHP1 cDNA sequence constructed in an RNAi-silencing vector was transferred to oilseed rape cv. SLM046. Molecular analysis of T2 transgenic plants by RT-PCR and Real-time PCR showed that expression of the BnSHP alleles was highly decreased in comparison with control plants. Morphologically, transgenic plants were normal and produced seeds at greenhouse conditions. At ripening, stage pods failed to shatter, and a finger pressure was needed for pod opening.