Culture of Meloidogyne incognita on oriental-melon roots genetically transformed by Agrobacterium rhizogenes

The root-knot nematode, Meloidogyne incognita, was cultured monoxenically on oriental-melon (Cucumis melo L.) roots transformed by Agrobacterium rhizogenes at various temperatures. The developmental zero degree of the nematode was 10.9°C. The day-degrees for the onset of oviposition were 293, and 47...

Full description

Saved in:
Bibliographic Details
Published inJapanese Journal of Applied Entomology and Zoology Vol. 36; no. 4; pp. 225 - 230
Main Authors ADACHI, Hiroshi, NARABU, Takashi, MOMOTA, Yoji
Format Journal Article
LanguageJapanese
English
Published Tokyo JAPANESE SOCIETY OF APPLIED ENTOMOLOGY AND ZOOLOGY 1992
Japanese Society of Applied Entomology and Zoology
Japan Science and Technology Agency
Subjects
Online AccessGet full text

Cover

Loading…
More Information
Summary:The root-knot nematode, Meloidogyne incognita, was cultured monoxenically on oriental-melon (Cucumis melo L.) roots transformed by Agrobacterium rhizogenes at various temperatures. The developmental zero degree of the nematode was 10.9°C. The day-degrees for the onset of oviposition were 293, and 473 for completing one generation after inoculation with 2nd-stage juveniles. There were no significant differences in the dimensions of eggs and pre-parasitic 2nd-stage juveniles between transformed roots and intact plant roots, although a slight temperature-dependency was seen in body length, tail length, and the a-avalue of juveniles. Regarding the infectivity on six differential host plants, juveniles produced on transformed roots were the same as those produced on intact host plant roots. These results suggest that the transformed root system is appropriate for culture of root-knot nematodes under sterile conditions.
Bibliography:H
H10
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0021-4914
1347-6068
DOI:10.1303/jjaez.36.225