Effect of Type 1 Herpes Simplex Infection of Phenotypic Peculiarities of Human Vascular Endothelial Cells in Culture

• Published in: Bulletin of experimental biology and medicine Vol. 155; no. 1; pp. 151 - 158
• Main Authors: Scheglovitova, O. N., Skliankina, N. N., Boldyreva, N. V., Babayants, A. A., Frolova, I. S.
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.05.2013; Springer; Springer Nature B.V
• Subjects: Antigens, CD - metabolism; Biomedical and Life Sciences; Biomedicine; Cadherins - metabolism; Cell Biology; Cells, Cultured; E-Selectin - metabolism; Endothelial Cells - immunology; Endothelial Cells - metabolism; Endothelial Cells - virology; Endothelin-1 - metabolism; Endothelium; Health aspects; Herpes simplex; Herpesvirus 1, Human - immunology; Humans; Infection; Interleukin-1beta - metabolism; Interleukin-6 - metabolism; Interleukin-8 - metabolism; Internal Medicine; Laboratory Medicine; Nitric Oxide - metabolism; P-Selectin - metabolism; Pathology; Platelet Endothelial Cell Adhesion Molecule-1 - metabolism; Tumor necrosis factor; Tumor Necrosis Factor-alpha - metabolism; Vasodilators; Virus diseases; vascular endothelium; cytokines; type 1 herpes simplex virus; inflammation; shedding of cell adhesion molecules
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-013-2101-9

We studied the effect of type 1 herpes simplex infection on the production of innate immunity mediators in human vascular endothelial cells in culture. It was found that production of anti-inflammatory cytokines IL-1β, IL.6, and TNF-α in infected cultures depended on the level of their spontaneous production, while IL-8 production was suppressed irrespective of its spontaneous level. Shedding of cell adhesion molecules of early (P-selectin and E-selectin) and late (PECAM-1 and VE-cadherin) phases of leukocyte recruitment depended on individual capacity of human vascular endothelial cell cultures to maintain reproduction of type 1 herpes simples virus. The production of vasodilator NO and vasoconstrictor endothelin-1 by infected cultures also depended on spontaneous synthesis of this transmitter by non-infected cultures.