Detection of arthritogenic factor in adjuvant arthritis

• Published in: The Journal of immunology (1950) Vol. 140; no. 6; pp. 1838 - 1843
• Main Authors: Helfgott, SM, Kieval, RI, Breedveld, FC, Brahn, E, Young, CT, Dynesius-Trentham, R, Trentham, DE
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Am Assoc Immnol 15.03.1988; American Association of Immunologists
• Subjects: Animals; Arthritis - metabolism; Arthritis, Experimental - metabolism; Biological and medical sciences; Cartilage - physiopathology; Cell Line; Collagen - metabolism; Diamines - toxicity; Diseases of the osteoarticular system; Female; Freund's Adjuvant - toxicity; Inflammatory joint diseases; Lymphokines - isolation & purification; Lymphokines - metabolism; Lymphokines - toxicity; Male; Medical sciences; Mice; Mice, Inbred DBA; Rats; Rats, Inbred Lew; Rats, Inbred WF; Synovitis - chemically induced; T-Lymphocytes - analysis; Immunopathology; Rat; Induction; Rodentia; Diseases of the osteoarticular system; Arthritis; Lymphokine; Inflammatory joint disease; Characterization; Vertebrata; Experimental disease; Mammalia; Freund's adjuvant; Immunological adjuvant; Animal; Collagen
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.140.6.1838

An evocation of arthritis by an Ag-specific lymphokine has recently been considered with the description of arthritogenic factor (AF) in rats with collagen arthritis. Because rats with CFA-induced arthritis also exhibit T cell reactivity to native type II collagen, T cell lines specific for this protein were established from CFA-injected rats. Supernatant material from these lines contained a type II collagen-binding lymphokine with functional and biochemical attributes identical with those described for AF, i.e., it was a 65-kDa species cross-reacting immunoprotein possessing the ability to incite an erosive, proliferative synovitis when injected into the knee joint of naive recipients. Similarities were also observed with HPLC and on two-dimensional gels. Lymph node cells from rats with arthritis created by injection of the synthetic adjuvant, CP-20,961 failed to produce AF, suggesting that this material is not a ubiquitous concomitant of inflammatory arthritis in the rat. Test injections into sites contiguous with the ear cartilage plate and into fibroblast-lined s.c. pouches suggested that cartilage was a requisite for the induction of inflammation by AF. These data identify a potentially shared effector pathway in the collagen and adjuvant models. The presence of AF in two frequently used models further supports the hypothesis that Ag-specific lymphokines can create autoimmune disease.