First-dose and steady-state pharmacokinetics of orally administered crizotinib in children with solid tumors: a report on ADVL0912 from the Children’s Oncology Group Phase 1/Pilot Consortium

• Published in: Cancer chemotherapy and pharmacology Vol. 79; no. 1; pp. 181 - 187
• Main Authors: Balis, Frank M., Thompson, Patrick A., Mosse, Yael P., Blaney, Susan M., Minard, Charles G., Weigel, Brenda J., Fox, Elizabeth
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.01.2017; Springer Nature B.V
• Subjects: Administration, Oral; Adolescent; Adult; Cancer Research; Child; Child, Preschool; Female; Humans; Lymphoma, Large-Cell, Anaplastic - drug therapy; Male; Medicine; Medicine & Public Health; NCT; NCT00939770; Neoplasms - drug therapy; Oncology; Original; Original Article; Pharmacology/Toxicology; Protein Kinase Inhibitors - administration & dosage; Protein Kinase Inhibitors - pharmacokinetics; Pyrazoles - administration & dosage; Pyrazoles - pharmacokinetics; Pyridines - administration & dosage; Pyridines - pharmacokinetics; Crizotinib; ALK; Children; Pharmacokinetics; Childhood cancer
• ISSN: 0344-5704; 1432-0843
• DOI: 10.1007/s00280-016-3220-6

Purpose Characterize the pharmacokinetics of oral crizotinib in children with cancer. Methods Sixty-four children with solid tumors or anaplastic large-cell lymphoma (ALCL) enrolled on a phase 1/2 trial of the ALK, MET and ROS1 inhibitor, crizotinib, had pharmacokinetic sampling after the first dose ( n  = 15) or at steady state ( n  = 49). Dose levels studied were 100, 130, 165, 215, 280 and 365 mg/m 2 /dose administered twice daily. Two capsule and two oral liquid formulations were used over the course of the trial. Crizotinib was quantified with a validated HPLC/tandem mass spectrometry method with a lower limit of detection of 0.2 ng/mL. Pharmacokinetic parameters were derived using non-compartmental analysis. Results Time to peak plasma concentration was 4 h. At 280 mg/m 2 (MTD), mean (±SD) steady-state peak plasma concentration was 717 ± 201 ng/mL, and steady-state trough plasma concentration was 480 ± 176 ng/mL. At steady state, AUC 0– τ was proportional to dose over the dose range of 215–365 mg/m 2 /dose. Apparent clearance of crizotinib was 731 ± 241 mL/min/m 2 . Steady-state AUC 0– τ at 280 mg/m 2 /dose was 2.5-fold higher than the AUC 0– ∞ in adults receiving 250 mg (~140 mg/m 2 ). Age, sex and drug formulation do not account for the inter-subject variability in AUC 0– τ at steady state. The accumulation index was 4.9, and the half-life estimated from the accumulation index was 36 h. Conclusions The pharmacokinetics of oral crizotinib in children is similar to that in adults. Steady-state trough-free crizotinib concentrations in plasma at the MTD exceed inhibitory concentrations of crizotinib in ALCL cell lines. ClinicalTrials.gov identifier NCT00939770.