Molecular identification and genetic diversity among Photorhabdus and Xenorhabdus isolates
Five bacterial strains were isolated from the hemocoel of the greater wax moth larvae ( Galleria mellonella ) infected with the entomopathogenic nematodes: Heterorhabditis bacteriophora HP88, Heterorhabditis indicus RM1 and Heterorhabditis sp (S1), Steinernema abbasi and Steinernema sp. (S II). Stra...
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Published in | 3 Biotech Vol. 7; no. 1; pp. 6 - 9 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer Berlin Heidelberg
01.05.2017
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Five bacterial strains were isolated from the hemocoel of the greater wax moth larvae (
Galleria mellonella
) infected with the entomopathogenic nematodes:
Heterorhabditis bacteriophora
HP88,
Heterorhabditis indicus
RM1 and
Heterorhabditis sp
(S1),
Steinernema abbasi
and
Steinernema
sp. (S II). Strains were identified as
Photorhabdus luminescens
HRM1,
P. luminescens
HS1,
P. luminescens
HP88,
Xenorhabdus indica
and
X. nematophila
ATTC19061 using 16S rDNA sequence analysis. To reveal the genetic diversity among these strains, three molecular markers (RAPD, ISSR and SRAP) were employed. RAPD analysis showed 73.8 and 54.5 polymorphism percentages for the
Photorhabdus
and
Xenorhabdus
strains, respectively. ISSR analysis resulted in 70.1 and 75.2 polymorphism percentages among the
Photorhabdus
and
Xenorhabdus
strains, respectively. The SRAP analysis indicated that 75.6 and 61.2% genetic polymorphism was detected among
Photorhabdus
and Xenorhabdus strains, respectively. The cluster analysis grouped the three
Photorhabdus strains
together in one cluster and the two
Xenorhabdus
strains together in another cluster indicating the phylogenetic relationships among them. The genotype-specific markers detected from the three molecular markers (RAPD, ISSR and SRAP) were sufficient to distinguish between the different bacterial strains tested and can be used in the future IBM program that could be built on the use of these strains. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2190-572X 2190-5738 |
DOI: | 10.1007/s13205-016-0594-4 |