MEDUSA: A cloud-based tool for the analysis of X-ray diffuse scattering to obtain the bending modulus from oriented membrane stacks

An important mechanical property of cells is their membrane bending modulus, κ. Here, we introduce MEDUSA (MEmbrane DiffUse Scattering Analysis), a cloud-based analysis tool to determine the bending modulus, κ, from the analysis of X-ray diffuse scattering. MEDUSA uses GPU (graphics processing unit)...

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Published inPLoS computational biology Vol. 20; no. 1; p. e1011749
Main Authors Himbert, Sebastian, Gaboo, Dorian, Brookes, Emre, Nagle, John F, Rheinstädter, Maikel C
Format Journal Article
LanguageEnglish
Published United States Public Library of Science 01.01.2024
Public Library of Science (PLoS)
Subjects
Algorithms
Bending modulus
Cells
Cloud Computing
Graphical user interface
Graphics processing units
Humidity
Lipids
Mechanical properties
Membranes
Radiography
Scattering
Software
Technology application
X-Rays
Canada
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Summary:An important mechanical property of cells is their membrane bending modulus, κ. Here, we introduce MEDUSA (MEmbrane DiffUse Scattering Analysis), a cloud-based analysis tool to determine the bending modulus, κ, from the analysis of X-ray diffuse scattering. MEDUSA uses GPU (graphics processing unit) accelerated hardware and a parallelized algorithm to run the calculations efficiently in a few seconds. MEDUSA's graphical user interface allows the user to upload 2-dimensional data collected from different sources, perform background subtraction and distortion corrections, select regions of interest, run the fitting procedure and output the fitted parameters, the membranes' bending modulus κ, and compressional modulus B.
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ISSN:1553-7358
1553-734X
1553-7358
DOI:10.1371/journal.pcbi.1011749