Phylogeny and classification of Odonata using targeted genomics

• Published in: Molecular phylogenetics and evolution Vol. 160; p. 107115
• Main Authors: Bybee, Seth M., Kalkman, Vincent J., Erickson, Robert J., Frandsen, Paul B., Breinholt, Jesse W., Suvorov, Anton, Dijkstra, Klaas-Douwe B., Cordero-Rivera, Adolfo, Skevington, Jeffrey H., Abbott, John C., Sanchez Herrera, Melissa, Lemmon, Alan R., Moriarty Lemmon, Emily, Ware, Jessica L.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.07.2021
• Subjects: Anchored hybrid enrichment; Damselfly; Dragonfly; Evolution; genomics; hybrids; insects; introgression; monophyly; Quartet sampling; Taxonomy; topology; Zygoptera; Evolution; Quartet sampling; Dragonfly; Damselfly; Anchored hybrid enrichment; Taxonomy
• ISSN: 1055-7903; 1095-9513; 1095-9513
• DOI: 10.1016/j.ympev.2021.107115

•A set of anchor hybridization probes are developed for all lineages of Odonata.•Phylogenetic reconstruction of all but two major odonate lineages.•An improved phylogenetic backbone of Odonata is provided.•Five new families are proposed and two more reinstated.•An updated classification scheme for Odonata is provided. Dragonflies and damselflies are a charismatic, medium-sized insect order (~6300 species) with a unique potential to approach comparative research questions. Their taxonomy and many ecological traits for a large fraction of extant species are relatively well understood. However, until now, the lack of a large-scale phylogeny based on high throughput data with the potential to connect both perspectives has precluded comparative evolutionary questions for these insects. Here, we provide an ordinal hypothesis of classification based on anchored hybrid enrichment using a total of 136 species representing 46 of the 48 families or incertae sedis, and a total of 478 target loci. Our analyses recovered the monophyly for all three suborders: Anisoptera, Anisozygoptera and Zygoptera. Although the backbone of the topology was reinforced and showed the highest support values to date, our genomic data was unable to stronglyresolve portions of the topology. In addition, a quartet sampling approach highlights the potential evolutionary scenarios that may have shaped evolutionary phylogeny (e.g., incomplete lineage sorting and introgression) of this taxon. Finally, in light of our phylogenomic reconstruction and previous morphological and molecular information we proposed an updated odonate classification and define five new families (Amanipodagrionidae fam. nov., Mesagrionidae fam. nov., Mesopodagrionidae fam. nov., Priscagrionidae fam. nov., Protolestidae fam. nov.) and reinstate another two (Rhipidolestidae stat. res., Tatocnemididae stat. res.). Additionally, we feature the problematic taxonomic groupings for examination in future studies to improve our current phylogenetic hypothesis.