A Simple and Selective Fluorescent Sensor Chip for Indole-3-Butyric Acid in Mung Bean Sprouts Based on Molecularly Imprinted Polymer Coatings

In this paper, we report the preparation of molecularly imprinted polymer coatings on quartz chips for selective solid-phase microextraction and fluorescence sensing of the auxin, indole-3-butyric acid. The multiple copolymerization method was used to prepare polymer coatings on silylated quartz chi...

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Bibliographic Details
Published inSensors (Basel, Switzerland) Vol. 17; no. 9; p. 1954
Main Authors Chang, Jiahua, Bahethan, Bota, Muhammad, Turghun, Yakup, Burabiye, Abbas, Mamatimin
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 24.08.2017
MDPI
Subjects
Beansprouts
Binding
Butyric acid
Chips
Condensed Matter
Copolymerization
Crosslinking
Fluorescence
fluorescence detection
indole-3-butyric acid
Materials Science
molecularly imprinted polymer
Physics
polymer coating
Polymer coatings
Polymers
Quartz
Selectivity
sensor chip
sensor chip
molecularly imprinted polymer
polymer coating
fluorescence detection
indole-3-butyric acid
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Summary:In this paper, we report the preparation of molecularly imprinted polymer coatings on quartz chips for selective solid-phase microextraction and fluorescence sensing of the auxin, indole-3-butyric acid. The multiple copolymerization method was used to prepare polymer coatings on silylated quartz chips. The polymer preparation conditions (e.g., the solvent, monomer, and cross-linker) were investigated systemically to enhance the binding performance of the imprinted coatings. Direct solid-phase fluorescence measurements on the chips facilitated monitoring changes in coating performance. The average binding capacity of an imprinted polymer coated chip was approximately 152.9 µg, which was higher than that of a non-imprinted polymer coated chip (60.8 µg); the imprinted coatings showed the highest binding to IBA among the structural analogues, indicating that the coatings possess high selectivity toward the template molecule. The developed method was used for the determination of the auxin in mung bean extraction, and the recovery was found to be in the range of 91.5% to 97.5%, with an RSD (n = 3) of less than 7.4%. Thus, the present study provides a simple method for fabricating a fluorescent sensor chip for selective analysis.
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PMCID: PMC5620659
ISSN:1424-8220
1424-8220
DOI:10.3390/s17091954