Nanopipettes as a Potential Diagnostic Tool for Selective Nanopore Detection of Biomolecules

Nanopipettes, as a class of solid-state nanopores, have evolved into universal tools in biomedicine for the detection of biomarkers and different biological analytes. Nanopipette-based methods combine high sensitivity, selectivity, single-molecule resolution, and multifunctionality. The features hav...

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Published inBiosensors (Basel) Vol. 14; no. 12; p. 627
Main Authors Kuanaeva, Regina M., Vaneev, Alexander N., Gorelkin, Petr V., Erofeev, Alexander S.
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 01.12.2024
MDPI
Subjects
Biochemistry
Biocompatibility
Biology
Biomarkers
Biomarkers - analysis
Biomolecules
Biosensing Techniques
Biotechnology
Blood vessels
Charged particles
Diagnostic software
Dielectric properties
DNA - analysis
Electric fields
Electrochemical Techniques
Electrochemistry
Humans
Lasers
Microscopy
molecular diagnostics
nanopipette
nanopore
Nanopores
Peptides
Pore size
Portable equipment
Precision medicine
Proteins
Proteins - analysis
Review
RNA
RNA - analysis
Selectivity
single molecule
solid-state nanopore
Synuclein
β-Amyloid
New Mexico
nanopipette
single molecule
molecular diagnostics
solid-state nanopore
nanopore
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Summary:Nanopipettes, as a class of solid-state nanopores, have evolved into universal tools in biomedicine for the detection of biomarkers and different biological analytes. Nanopipette-based methods combine high sensitivity, selectivity, single-molecule resolution, and multifunctionality. The features have significantly expanded interest in their applications for the biomolecular detection, imaging, and molecular diagnostics of real samples. Moreover, the ease of manufacturing nanopipettes, coupled with their compatibility with fluorescence and electrochemical methods, makes them ideal for portable point-of-care diagnostic devices. This review summarized the latest progress in nanopipette-based nanopore technology for the detection of biomarkers, DNA, RNA, proteins, and peptides, in particular β-amyloid or α-synuclein, emphasizing the impact of technology on molecular diagnostics. By addressing key challenges in single-molecule detection and expanding applications in diverse biological areas, nanopipettes are poised to play a transformative role in the future of personalized medicine.
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These authors contributed equally to this work.
ISSN:2079-6374
2079-6374
DOI:10.3390/bios14120627