MicroRNA-148a Regulates the Proliferation and Differentiation of Ovine Preadipocytes by Targeting PTEN

• Published in: Animals (Basel) Vol. 11; no. 3; p. 820
• Main Authors: Jin, Xiayang, Hao, Zhiyun, Zhao, Mengli, Shen, Jiyuan, Ke, Na, Song, Yize, Qiao, Lirong, Lu, Yujie, Hu, Liyan, Wu, Xinmiao, Wang, Jiqing, Luo, Yuzhu
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 15.03.2021; MDPI
• Subjects: Adipocytes; Adipogenesis; Adipose tissue; Cell cycle; Cholecystokinin; Cyclin-dependent kinases; Deoxyribonucleic acid; Differentiation; DNA; Fatty acids; Flow cytometry; Fluorescence; Genes; Investigations; Kinases; Laboratory animals; Lipid metabolism; Lipids; Meat quality; MicroRNAs; miR-148a; miRNA; Overexpression; ovine preadipocytes; Preadipocytes; proliferation; PTEN; PTEN protein; Sheep; Spleen; Staining; Tumorigenesis; Beijing China; United States > US; China; Japan; PTEN; adipogenesis; proliferation; ovine preadipocytes; miR-148a
• ISSN: 2076-2615; 2076-2615
• DOI: 10.3390/ani11030820

MicroRNAs (miRNAs) have been found to be involved in lipid deposition and metabolism. However, there have been no reports on the roles of miR-148a in the proliferation and adipogenesis of preadipocytes in sheep. In this study, the expression of miR-148a was profiled in the eight tissues of Tibetan ewes and differentiated preadipocytes, and the role of miR-148a in differentiation and proliferation of ovine preadipocytes was investigated using Oil Red O staining, CCK-8, EdU staining, cell cycle detection, and RT-qPCR. The effect of on the differentiation of ovine preadipocytes was also investigated. The miR-148a was widely expressed in the eight tissues investigated and had significantly increased expression in liver, spleen and subcutaneous adipose tissues, and the heart. The expression of miR-148a continued to increase with the differentiation of ovine preadipocytes. The over-expression of miR-148a significantly promoted differentiation but inhibited the proliferation of ovine preadipocytes. The inhibition of miR-148a had the opposite effect on the differentiation and proliferation of ovine preadipocytes with over-expressed miR-148a. The results from the dual luciferase reporter assays showed that miR-148a mimic significantly decreased the luciferase activity of -3'UTR dual luciferase reporter vector, suggesting that is a target gene of miR-148a. In over-expressed- preadipocytes, the number of lipid droplets remarkably decreased, and the expression levels of adipogenesis marker genes , , , , and were also significantly down-regulated. These results suggest that miR-148a accelerated the adipogenic differentiation of ovine preadipocytes by inhibiting expression, and also inhibited the proliferation of ovine preadipocytes.