The use of multifunctional magnetic mesoporous core/shell heteronanostructures in a biomolecule separation system

• Published in: Biomaterials Vol. 32; no. 21; pp. 4683 - 4690
• Main Authors: Liu, Zhen, Li, Meng, Yang, Xinjian, Yin, Meili, Ren, Jinsong, Qu, Xiaogang
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.07.2011
• Subjects: Advanced Basic Science; Bionanotechnology; Dentistry; Escherichia coli; Escherichia coli - chemistry; Escherichia coli Proteins - isolation & purification; Heteronanostructure; Humans; Iron - chemistry; Magnetite Nanoparticles; Materials Testing; Mesoporous material; Nickel - chemistry; Particle Size; Peptide enrichment; Porosity; Protein separation; Serum Albumin - isolation & purification; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; Ultrasonics; Protein separation; Heteronanostructure; Mesoporous material; Bionanotechnology; Peptide enrichment
• ISSN: 0142-9612; 1878-5905
• DOI: 10.1016/j.biomaterials.2011.03.038

Abstract A multifunctional magnetic mesoporous core/shell heteronanostructure (designated as Fe3 O4 @NiSiO3 ) has been designed and constructed that combined the capacity of effective protein purification from protein mixture and selective low molecule weight (MW) biomolecule enrichment. The nanoparticle is composed by magnetite nanoparticle with immobilized metal ion surface and solid porous shell which presents a number of important features, such as controllable shell thickness, uniform pore size and excellent magnetism. By taking advantages of the high affinity of Ni2+ on the shell surface toward His-tagged proteins and the fast response toward an assistant magnet, the heteronanoparticles can be applied to selectively bind to and magnetically separate of His-tagged proteins from a cell lysate of E. coli . Additionally, owing to the homogeneous 3D mesopores on the nickel silicate shell, the heteronanoparticles can selectively capture low MW biomolecules from complex mixture. Significantly, it is expected that this approach can be extended to other biomolecule separation and enrichment systems by changing the immobilized surface and the pore size.