Molecular discrimination among taxa of Petunia axillaris complex and P. integrifolia complex based on PolA1 sequence analysis

Petunia axillaris complex and P. integrifolia complex consist of three subspecies (ssp. axillaris, ssp. parodii and ssp. subandina) and two species (P. integrifolia and P. inflata), respectively. Since these taxa within each complex can only be distinguished by the flower morphology, it would be use...

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Bibliographic Details
Published inBreeding Science Vol. 58; no. 1; pp. 71 - 75
Main Authors Zhang, Xinwang, Takahashi, Hiroko, Nakamura, Ikuo, Mii, Masahiro
Format Journal Article
LanguageEnglish
Published Tokyo Japanese Society of Breeding 2008
Japan Science and Technology Agency
Subjects
discrimination
DNA marker
Petunia
PolA1
sequence analysis
Solanaceae
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Summary:Petunia axillaris complex and P. integrifolia complex consist of three subspecies (ssp. axillaris, ssp. parodii and ssp. subandina) and two species (P. integrifolia and P. inflata), respectively. Since these taxa within each complex can only be distinguished by the flower morphology, it would be useful to discriminate them by DNA markers. In this study, we sequenced the 19th intron and the 20th exon of the PolA1 gene, a single gene encoding the largest subunit of RNA polymerase I, and showed clear differences between P. axillaris complex and P. integrifolia complex, and also among all the taxa within each complex. These sequence variations will be promising to develop PCR-based markers to discriminate taxa within P. axillaris complex and P. integrifolia complex.
ISSN:1344-7610
1347-3735
DOI:10.1270/jsbbs.58.71