Effects of antioxidative agents on apoptosis induced by ischaemia‐reperfusion in rat intestinal mucosa

• Published in: Alimentary pharmacology & therapeutics Vol. 18; no. s1; pp. 139 - 145
• Main Authors: Kojima, M., Iwakiri, R., Wu, B., Fujise, T., Watanabe, K., Lin, T., Amemori, S., Sakata, H., Shimoda, R., Oguzu, T., Ootani, A., Tsunada, S., Fujimoto, K.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.07.2003; Blackwell
• Subjects: Alanine - analogs & derivatives; Alanine - pharmacology; Animals; Anti-Ulcer Agents - pharmacology; Antioxidants - pharmacology; Apoptosis - drug effects; Biological and medical sciences; Dimethyl Sulfoxide - pharmacology; Free Radical Scavengers - pharmacology; Gastroenterology. Liver. Pancreas. Abdomen; Glutathione - pharmacology; Immunohistochemistry; Intestinal Mucosa; Male; Medical sciences; Other diseases. Semiology; Quinolones - pharmacology; Rats; Rats, Sprague-Dawley; Reperfusion Injury - prevention & control; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus; Oxidative stress; Rat; Pathogenesis; Mucosa; Cardiovascular disease; Free radical; Reperfusion; Ischemia; Intestinal disease; Rebamipide; Glutathione; Oxygen; Rodentia; Gut; Quinoline derivatives; Antioxidant; α-Aminoacid; Vertebrata; Chemotherapy; Mammalia; Treatment; Cell death; Animal; Dimethyl sulfoxide; Digestive diseases; Apoptosis
• ISSN: 0269-2813; 1365-2036
• DOI: 10.1046/j.1365-2036.18.s1.16.x

Summary Background & aim : We have previously demonstrated that ischaemia‐reperfusion induces apoptosis in the intestinal mucosa. To evaluate that reactive oxygen species enhanced intestinal apoptosis after ischaemia‐reperfusion, we examined whether antioxidants reduced apoptosis. Methods : Rats were infused through a duodenal tube with antioxidative agents, glutathione, rebamipide and dymethylsulfoxide during 2 h before an ischaemic insult. The superior mesenteric artery was occluded for 60 min, followed by 60 min reperfusion. Apoptosis was evaluated by percentage fragmented DNA (fragmented DNA/total DNA) and immunochemical staining. Results : Increase in apoptosis in the intestinal mucosa after ischaemia‐reperfusion was attenuated by intraduodenal infusion of antioxidative agents, but was not completely abolished. Conclusion : Scavenging effects of the antioxidative agents attenuated increases in intestinal apoptosis, indicating that oxidative stress after ischaemia‐reperfusion plays an important role in induction of apoptosis in the intestinal mucosa.