The microtubule end‐binding protein FgEB1 regulates polar growth and fungicide sensitivity via different interactors in Fusarium graminearum

• Published in: Environmental microbiology Vol. 19; no. 5; pp. 1791 - 1807
• Main Authors: Liu, Zunyong, Wu, Sisi, Chen, Yun, Han, Xinyue, Gu, Qin, Yin, Yanni, Ma, Zhonghua
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01.05.2017
• Subjects: actin; Benzimidazoles - pharmacology; Biosynthesis; Carbamates - pharmacology; carbendazim; Carrier Proteins - genetics; Carrier Proteins - metabolism; cell polarity; Cell Polarity - genetics; chromosomes; conidia; deoxynivalenol; Fungal Proteins - genetics; Fungal Proteins - metabolism; Fungicides; Fungicides, Industrial - pharmacology; Fusarium - drug effects; Fusarium - genetics; Fusarium - growth & development; Fusarium graminearum; Gene Deletion; hyphae; Hyphae - growth & development; Microbial Sensitivity Tests; microscopy; Microtubule-Associated Proteins - genetics; Microtubule-Associated Proteins - metabolism; microtubules; Microtubules - genetics; mutants; myosin; Myosins; Nuclear Proteins - metabolism; Plant Diseases - microbiology; Septins - metabolism; Spores, Fungal - growth & development; Trichothecenes - biosynthesis; Triticum - microbiology; Virulence; Yeasts
• ISSN: 1462-2912; 1462-2920
• DOI: 10.1111/1462-2920.13651

Summary In yeasts, the end‐binding protein 1 (EB1) homologs regulate microtubule dynamics, cell polarization, and chromosome stability. However, functions of EB1 orthologs in plant pathogenic fungi have not been characterized yet. Here, we observed that the FgEB1 deletion mutant (ΔFgEB1) of Fusarium graminearum exhibits twisted hyphae, increased hyphal branching and curved conidia, indicating that FgEB1 is involved in the regulation of cellular polarity. Microscopic examination further showed that the microtubules of ΔFgEB1 exhibited less organized in comparison with those of the wild type. In addition, the lack of FgEB1 also altered the distribution of polarity‐related class I myosin via the interaction with the actin. On the other hand, we identified four core septins as FgEB1‐interacting proteins, and found that FgEB1 and septins regulated conidial polar growth in the opposite orientation. Interestingly, FgEB1 and FgKar9 constituted another complex that modulated the response to carbendazim, a microtubule‐damaging agent specifically. In addition, the deletion of FgEB1 led to dramatically decreased deoxynivalenol (DON) biosynthesis. Taken together, results of this study indicate that FgEB1 regulates cellular polarity, fungicide sensitivity and DON biosynthesis via different interactors in F. graminarum, which provides a novel insight into understanding of the biological functions of EB1 in filamentous fungi.