Detection of human papillomavirus DNA in benign oral squamous epithelial lesions in Venezuela

• Published in: Journal of oral pathology & medicine Vol. 30; no. 7; pp. 385 - 388
• Main Authors: Jimenez, C., Correnti, M., Salma, N., Cavazza, M. E., Perrone, M.
• Format: Journal Article
• Language: English
• Published: Copenhagen Munksgaard International Publishers 01.08.2001; Blackwell
• Subjects: Adolescent; Adult; Aged; Biological and medical sciences; Biopsy; buccal mucosa; Child; Child, Preschool; Condylomata Acuminata - virology; Dentistry; DNA Primers; DNA, Viral - analysis; Female; Focal Epithelial Hyperplasia - virology; Genome, Viral; human papillomavirus; Humans; Male; Medical sciences; Middle Aged; Mouth Diseases - virology; Mouth Neoplasms - virology; oral benign lesions; Otorhinolaryngology. Stomatology; Papilloma - virology; Papillomaviridae - classification; Papillomaviridae - genetics; Papillomavirus Infections - diagnosis; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Risk Factors; Tumor Virus Infections - diagnosis; Tumors; Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology; Venezuela; Warts - virology; South America; Venezuela; America; Human; Stomatology; Papovaviridae; Epidemiology; Incidence; Papillomavirus; Virus; Infection; Polymerase chain reaction; Oral cavity; Human papillomavirus; DNA; Oral cavity disease; Lesion; Benign neoplasm; Molecular biology; Detection
• ISSN: 0904-2512; 1600-0714
• DOI: 10.1034/j.1600-0714.2001.300701.x

: The polymerase chain reaction (PCR) was applied to the detection of human papillomavirus (HPV) infection in biopsies taken from clinically normal oral mucosa of 20 subjects and clinical lesions of 40 patients. PCR for HPV‐DNA amplification was performed using consensus primers MYO9/MYO11 and subsequent typing for HPV of high and low oncogenic risk HPV types were identified by restriction enzyme analysis (restriction fragment length polymorphism, RFLP). The HPV viral genome was present in 55% (22/40) of the oral benign lesions (OBL) and in 10% (2/20) of the control samples. In the PCR+ OBL, we observed 90.9% of low oncogenic risk types (HPV‐6 ‐13 and ‐32) and 9.1% of the samples had a mixed infection with low and high oncogenic types (HPV‐6 and ‐16). ln the control samples, we observed one patient with HPV‐6 and another with HPV‐6 and ‐16 in the same sample. All of the eight focal epithelial hyperplasia cases were positive for low risk HPV types (88% HPV‐13 and 12.5% HPV‐32). In conclusion, this study demonstrates a high incidence of HPV in oral benign lesions from Venezuelan patients.