response of Chironomidae to sediment pollution and other environmental characteristics in urban wetlands

• Published in: Freshwater biology Vol. 52; no. 12; pp. 2444 - 2462
• Main Authors: CAREW, MELISSA E, PETTIGROVE, VINCENT, COX, RENEE L, HOFFMANN, ARY A
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.12.2007; Blackwell Publishing Ltd
• Subjects: bioindicators; Chironomidae; Chironomus; DNA identification; Freshwater; indicator species; polymerase chain reaction-restriction fragment length polymorphism; Tanytarsus; urban aquatic ecosystems; Australia, Victoria, Melbourne; Australia
• ISSN: 0046-5070; 1365-2427
• DOI: 10.1111/j.1365-2427.2007.01840.x

1. We investigated the distribution of chironomid taxa in urban wetlands in the greater Melbourne area, Australia, to test if their distribution was influenced by sediment pollution and other environmental variables. 2. For identification of the Chironomidae, DNA markers generated via polymerase chain reaction-restriction fragment length polymorphism of cytochrome c oxidase sub unit I (COI) were validated against morphology and reference specimens for more than 5000 chironomids representing over 80 species. DNA-based identification generally concurred with morphological separation, but also indicated the existence of cryptic diversity in some genera. 3. Non-metric multidimensional scaling (NMS) and canonical correspondence analysis (CCA) showed chironomid assemblages were structured among wetlands and could be linked to several habitat characteristics. However, Chironomidae assemblages were only weakly linked to sediment pollution. 4. Logistic regressions identified potential bioindicators of sediment pollution. Riethia stictoptera, Tanytarsus inextentus, Coelopynia and Chironomus 'februarius' were negatively associated and Chironomus duplex was positively associated with sediment pollution. Thresholds for the pollution sensitivities of specific species were mostly similar to those established with previous microcosm tests. 5. Several other environmental factors influenced the distribution of specific chironomid taxa. Salinity, substratum type and submerged and riparian vegetation were particularly important. 6. We conclude that specific chironomid taxa rather than assemblages have potential as bioindicators of sediment pollution provided their ecological preferences are considered and their pollution sensitivities are characterized using multiple methods. The integration of DNA-based techniques should facilitate accurate and rapid identification of bioindicators species.