The ecotoxicological evaluation of Cylindrospermopsis raciborskii from Lake Balaton (Hungary) employing a battery of bioassays and chemical screening

Ecotoxicity of four Cylindrospermopsis raciborskii strains (ACT 9502, ACT 9503, ACT 9504, ACT 9505) isolated from Lake Balaton (Hungary) was evaluated in four aquatic bioassays including the Thamnocephalus platyurus acute lethality test; Daphnia magna acute immobilization assay; D. magna feeding inh...

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Published inToxicon (Oxford) Vol. 70; pp. 98 - 106
Main Authors Ács, András, Kovács, Attila W., Csepregi, Janka Zsófia, Törő, Norbert, Kiss, Gyula, Győri, János, Vehovszky, Ágnes, Kováts, Nora, Farkas, Anna
Format Journal Article
LanguageEnglish
Published England Elsevier Ltd 01.08.2013
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Summary:Ecotoxicity of four Cylindrospermopsis raciborskii strains (ACT 9502, ACT 9503, ACT 9504, ACT 9505) isolated from Lake Balaton (Hungary) was evaluated in four aquatic bioassays including the Thamnocephalus platyurus acute lethality test; Daphnia magna acute immobilization assay; D. magna feeding inhibition assay and Danio rerio embryo developmental toxicity assay, assisted by chemical screening for known toxins by HPLC-MS. For reference, we analyzed in parallel the toxin content and toxic effects of two previously characterized toxin-producing strains: the Australian cylindrospermopsin producer AQS C. raciborskii and the anatoxins producer Oscillatoria sp. PCC 6506. Bioassays were used to evaluate the overall toxicity of the hydrophilic bioactive metabolites pool synthesized by the selected cyanobacteria. Chemical screening has proven that the ACT C. raciborskii extracts investigated did not contained cylindrospermopsins and anatoxins. The relative toxicity of the ACT C. raciborskii aqueous extracts observed in each bioassay was comparable to the effects recorded for the anatoxins producer PCC 6506 strain while toxicity values (EC50/LC50) calculated for the AQS extract were in general one order of magnitude lower. Concerning sublethal effects of ACT C. raciborskii extracts to the D. rerio embryogenesis, the general morphological abnormality observed was a significant retardation of development. Overall, our results suggest that C. raciborskii populating Lake Balaton produce metabolites with significant bioactive potencies. Therefore, continued investigation of these unknown compounds is required. •ACT Cylindrospermopsis raciborskii elicited significant feeding inhibition and lethality to crustacean neonates.•ACT C. raciborskii exerted significant retardation of Danio rerio embryogenesis.•ACT C. raciborskii do not synthesize cylindrospermopsins, anatoxins and saxitoxins.•Ecotoxicity risks to aquatic biota of present day maximal densities of C. raciborskii in Lake Balaton are moderate.
Bibliography:http://dx.doi.org/10.1016/j.toxicon.2013.04.019
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0041-0101
1879-3150
DOI:10.1016/j.toxicon.2013.04.019